FastQCFastQC Report
Tue 27 Jun 2023
SRR1041228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10340736
Total Bases527.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG8443798.165559975614888No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT729320.7052882889573817No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT649680.6282724943369601No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC546710.5286954429549309No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC500400.48391139663559735No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT490790.47461805426615666No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT441860.42730033916347926No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA404140.39082324507655936No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA396980.3838991731342914No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT357210.34543962828177804No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA344400.33305172861970367No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG313710.30337299008503843No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG278130.2689653811875673No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT274730.26567741406414397No Hit
CAGCCGGAATGTTAAGATCTTTAGTAATATTTTTTAAATTTGGACTATAC256340.24789338012303958No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT237840.2300029707750009No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA237030.22921966096030302No Hit
TACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGG235490.22773040526322305No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG218000.2108167155606719No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA188850.18262723272308665No Hit
CTATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATT185960.17983246066817682No Hit
TAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTA183690.17763725908871475No Hit
GGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAA180980.1750165558815156No Hit
GACGTGATATTAGTTTAATTCATTGTGAGATAGAATTGTTTATCTTGATA176400.1705874707564336No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA176270.17046175436642033No Hit
AGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAA167210.16170028903165112No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT161810.15647822360033173No Hit
ACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTA156070.15092736145667002No Hit
ATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTT155900.15076296310049883No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC153230.14818094185945757No Hit
TACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCGAACGCATAGCAC151420.14643058289081162No Hit
TACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCT146530.14170171252800573No Hit
ACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGGT134950.1305032833252875No Hit
AGTACTTATAGTAAGGTAAGTTTTACATCGATGTTGTATTCAGATAATCT134180.12975865547674748No Hit
AAATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCT122600.11856022627402925No Hit
TGTACTTTAATTGATGTTAATTATGAAATTATTATATTTTCTTTTAGATC121390.11739009679775211No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC120160.11620062633839602No Hit
TCCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGA113890.11013722814314185No Hit
GGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTGTT111670.10799037902137719No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT106240.10273930211543937No Hit
TTGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACC105050.10158851362224121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)