FastQCFastQC Report
Wed 28 Jun 2023
SRR1041236.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041236.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49915911
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG46584989.332691533967996No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT3416310.6844130321492079No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC2996590.6003276189830533No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT2939930.5889765289468522No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC2379490.47669970402824063No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT1980450.3967572584220691No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT1915700.3837854426817934No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA1570630.3146551807899489No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA1559040.31233327585667026No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG1531820.3068801048226887No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT1494790.2994616285777094No Hit
TACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGG1246350.24968992351957678No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA1193080.23901797565109048No Hit
CAGCCGGAATGTTAAGATCTTTAGTAATATTTTTTAAATTTGGACTATAC1181270.23665199659483327No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT1124570.225292893081727No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT1024760.20529726483405258No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG931560.18662586364496084No Hit
CTATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATT871920.1746777695793231No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA818900.16405590594149427No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT810770.1624271667605145No Hit
TAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTA805450.16136137433212427No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA798030.15987487436621162No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG785180.15730054491041945No Hit
GGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTGTT776440.1555496002066355No Hit
ATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTT770220.15430350454787853No Hit
GGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAA750120.15027673240302075No Hit
GACGTGATATTAGTTTAATTCATTGTGAGATAGAATTGTTTATCTTGATA729540.1461537985353007No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC705990.14143586400737032No Hit
ACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGGT699690.14017374139480296No Hit
AGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAA668660.1339572866856021No Hit
ACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTA656850.13159130762934487No Hit
TTGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACC647000.12961798894144194No Hit
TACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCT637260.12766670731502827No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA610870.12237981592682942No Hit
TGTACTTTAATTGATGTTAATTATGAAATTATTATATTTTCTTTTAGATC581760.11654800810907769No Hit
AAATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCT516550.10348403738439232No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC509460.10206364860294746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)