FastQCFastQC Report
Sun 2 Jul 2023
SRR1041238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG19496293.8992579999999997No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC2242240.448448No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT1495570.29911400000000005No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT1429270.285854No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG1280700.25614000000000003No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC1275330.255066No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA1264010.252802No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT985130.197026No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT939520.187904No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA896970.179394No Hit
TACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCGAACGCATAGCAC892010.178402No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT809950.16199No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC809060.161812No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA779910.155982No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA745430.149086No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT739950.14798999999999998No Hit
AGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAA716970.14339400000000002No Hit
GACGTGATATTAGTTTAATTCATTGTGAGATAGAATTGTTTATCTTGATA692580.138516No Hit
TAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTA690970.13819399999999998No Hit
TACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGG669400.13388No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG574350.11486999999999999No Hit
AGTACTTATAGTAAGGTAAGTTTTACATCGATGTTGTATTCAGATAATCT551050.11021No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT543020.10860399999999999No Hit
CTATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATT518390.10367799999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)