FastQCFastQC Report
Tue 27 Jun 2023
SRR1041244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11200297
Total Bases571.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG5046824.505969797050917No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT677630.605010742125856No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT523580.46746974656118495No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT360820.32215217150045217No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT303280.2707785338192371No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC279690.2497165923367925No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC275960.2463863235055285No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA237700.21222651506473444No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA217950.19459305409490482No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT203910.1820576722206563No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA199210.17786135492657024No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT185560.1656741781043842No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG182060.16254926097049033No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC147470.13166615135295073No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT146740.13101438292216716No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC146160.1304965395114076No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA144220.12876444258576358No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA142640.1273537657081772No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC139190.12427349024762468No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG125890.11239880513882802No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA120800.10785428279267952No Hit
GGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAA113640.1014615951701995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)