FastQCFastQC Report
Wed 28 Jun 2023
SRR1041245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG16358463.271692No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT2330800.46616No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT1756500.3513No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT1329010.265802No Hit
CAGGCAGTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAA1253340.250668No Hit
CAGCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGGTTTCTGTGGT1195070.23901399999999998No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC1171000.2342No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC1162000.23240000000000002No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT1129660.225932No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC966820.19336399999999998No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA891570.178314No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA845550.16911No Hit
ACTGCGAGTCAAAGGGTGTTAAAACCTTGCGGCGAAATGAAAGTAAAGGT843360.16867200000000002No Hit
GGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGCTTGAA836810.167362No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG832410.166482No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG785730.157146No Hit
GGAAACATCTGGAGAGAGGTTACCCGCACTCAAGTTCTCGCCAAGGTTTC763360.152672No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGCAACTATACCCC669840.133968No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA664710.132942No Hit
AGGCCATAAACCAGAGAGGGTGCGAGCCCCGTTCTGGATAGCGGCACTGT656940.131388No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT648060.129612No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT618810.12376200000000001No Hit
AAAGTGGGTGATAAACTTCATCTAAGGCTAAATATCGACTCGATTGCGAT549950.10999No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC545960.109192No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG537220.10744400000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)