FastQCFastQC Report
Tue 27 Jun 2023
SRR1041248.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041248.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084570
Total Bases106.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG709593.4040113788455173No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT90940.43625304019534No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT71840.3446274291580518No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT56080.26902430717126313No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC49820.23899413308260214No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT48030.23040723026811283No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA41360.19841022369121689No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC37310.17898175642938352No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC35580.17068268275951395No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA33300.1597451752639633No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG30190.1448260312678394No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT29920.14353080011705052No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA28940.13882959075492787No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT24500.1175302340530661No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG23960.11493977175148831No Hit
GGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGCTTGAA22040.10572923912365621No Hit
CAGCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGGTTTCTGTGGT21940.10524952388262328No Hit
CAGGCAGTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAA21150.10145977347846319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)