FastQCFastQC Report
Tue 27 Jun 2023
SRR1041249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49466571
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG18071093.653192375109243No Hit
GACAGATTGAAAGCTCTTTCTCGATTTGGTGGTTGGTGGTGCATGGCCGT2232170.4512481772791569No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT2151170.43487348253833885No Hit
CAGCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGGTTTCTGTGGT1854790.37495827232496065No Hit
CAGGCAGTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAA1790580.36197778900017147No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT1734630.3506671202254953No Hit
GGTTTGAGGTTGGGTTCGCCCTTCAACTGCCAGCAGGTTTACCTTGAATA1359550.2748421757392482No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT1281790.25912246878806294No Hit
GCCAGCAGGTTTACCTTGAATAAATCAGAGTGCTCAATACAAGCGCTTGC1206410.24388389484284242No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC1064950.21528680449671758No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT1023110.20682856711454692No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA954420.19294242166088285No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC944520.19094107008144956No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC936290.18927732023309238No Hit
ACTGCGAGTCAAAGGGTGTTAAAACCTTGCGGCGAAATGAAAGTAAAGGT885270.17896328411362897No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA793730.1604578574892527No Hit
TAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCT767100.15507442389730228No Hit
GGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGCTTGAA742570.15011551942826196No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG733800.14834260494829934No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG687010.138883691776412No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA670610.13556832148320935No Hit
GGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGG636680.12870914379733336No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT634340.1282360970603764No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGCAACTATACCCC613760.12407571165585746No Hit
AGAACTGATTTAATGGTTAAGAGGGACAAACCGGGGGCATTCGTATCATT581960.11764712779464741No Hit
AGGCCATAAACCAGAGAGGGTGCGAGCCCCGTTCTGGATAGCGGCACTGT580000.11725090061326467No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT557510.11270439586362274No Hit
TGCTTGCGGACGCTTTCTGGTGTGTGCTTGGACCTCGGTTCTAGTATCCT511740.10345168255143458No Hit
AGTTAAGGAACGGACTCCCTGGAATTGGTTCAGCCAGAGATGGGGACGTT511480.10339912180288383No Hit
TTCTGGTGTGTGCTTGGACCTCGGTTCTAGTATCCTGATCGCTCATCTAA506520.1023964244459152No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA497600.10059318645717326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)