FastQCFastQC Report
Tue 27 Jun 2023
SRR1041254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3724568
Total Bases189.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG1267363.4027033470727344No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT166820.4478908694914417No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT143370.3849305476500899No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT113160.30382046991758505No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC97270.26115780407284817No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC88140.23664489411926432No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA83130.2231936697088092No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT82660.2219317783968503No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT77320.2075945451928922No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA64880.17419469855295971No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT60130.1614415416767797No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC58670.15752162398431174No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG58540.15717259021717417No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA58310.1565550689368539No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC56480.1516417474456098No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT51910.13937186809315874No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG50950.13679438796660445No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC42740.11475156313430175No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA40290.1081736190613247No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA39280.10546189517817905No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT39190.10522025641631458No Hit
TACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCT37530.10076336369748116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)