FastQCFastQC Report
Mon 25 Sept 2023
SRR1041687.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041687.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA12095352.41907No Hit
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT7896591.579318RNA PCR Primer, Index 1 (100% over 25bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC7740811.548162No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA6053571.210714No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG5837821.1675639999999998No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC5317391.063478No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG4584580.9169160000000001No Hit
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA4496850.89937RNA PCR Primer, Index 1 (100% over 23bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA4485290.897058RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA3508720.7017439999999999No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG3454080.690816No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC3281070.656214RNA PCR Primer, Index 1 (100% over 24bp)
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC2963790.592758RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC2184890.43697800000000003RNA PCR Primer, Index 1 (100% over 24bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA2112250.42245RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1600870.32017399999999996No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGACTTCTCGGGTGCCA1493240.298648No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1234290.24685800000000002Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAAG1076600.21532No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAGG969750.19395Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA946490.189298RNA PCR Primer, Index 2 (100% over 40bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGCATTCTCGGGTGCC940920.18818400000000002No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA925060.185012RNA PCR Primer, Index 1 (100% over 22bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG909400.18188Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC897910.17958200000000002RNA PCR Primer, Index 1 (100% over 33bp)
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT859640.171928RNA PCR Primer, Index 2 (100% over 37bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA816410.163282No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA816020.163204No Hit
TTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTC775670.155134RNA PCR Primer, Index 1 (100% over 26bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA710860.142172RNA PCR Primer, Index 1 (100% over 22bp)
CTACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAA679060.135812No Hit
CTAAATCACTTGCATACGACTTGGTCTGGAATTCTCGGGTGCCAAGGAAC640080.128016RNA PCR Primer, Index 1 (100% over 24bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC636570.127314No Hit
CGGACGTTGACTGTCACTGATTTCTGCCCATGGAATTCTCGGGTGCCAAG634400.12688No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG584740.11694800000000001Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCAA579100.11582No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA572210.11444199999999999No Hit
TGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACTC568780.113756RNA PCR Primer, Index 1 (100% over 26bp)
CCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAGG564210.112842Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA523990.104798RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTTGGAATTCTCGGGTGCCAA520380.104076No Hit
CCCGGTGTCGGGAGGCATCTCTATCTCGTGTGGAATTCTCGGGTGCCAAG502720.10054400000000001No Hit
CTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACT500300.10006RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)