FastQCFastQC Report
Mon 25 Sept 2023
SRR1041690.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041690.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG11939242.387848No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG10863482.172696No Hit
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA8992621.798524RNA PCR Primer, Index 1 (100% over 23bp)
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC8030851.60617RNA PCR Primer, Index 1 (100% over 24bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA7410121.482024No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA7202361.440472No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAGG6250191.250038Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG6143561.228712No Hit
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA6088351.21767RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG4144950.8289899999999999Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA3676200.73524No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAAG2643870.528774No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2295690.459138Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG2177710.435542Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAA2085300.41706No Hit
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT1842820.368564RNA PCR Primer, Index 1 (100% over 25bp)
CTAAATCACTTGCATACGACTTGGTCTGGAATTCTCGGGTGCCAAGGAAC1611270.322254RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA1393310.278662RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1389610.277922No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC1362640.272528No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAGG1272230.254446Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGTTCGTGGCGGCTAGCGTTTATGGAATTCTCGGGTGCCAAGGAACT1263180.252636RNA PCR Primer, Index 1 (100% over 25bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA1174990.234998RNA PCR Primer, Index 1 (100% over 23bp)
CCCGGTGTCGGGAGGCATCTCTATCTCGTGTGGAATTCTCGGGTGCCAAG1090860.21817199999999998No Hit
CAGATCACTCTGGTTCAATGTCGGGGTGGAATTCTCGGGTGCCAAGGAAC1055540.211108RNA PCR Primer, Index 1 (100% over 24bp)
CTACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAA1030800.20615999999999998No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGTGGAATTCTCGGGTGCCAA1014910.202982No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGGTGGAATTCTCGGGTGCCA864730.172946No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA851380.170276No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAAG844640.168928No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC834210.16684200000000002RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGGA832500.1665RNA PCR Primer, Index 1 (100% over 22bp)
AGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCAA802740.160548No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGAA799030.159806RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC798960.15979200000000002RNA PCR Primer, Index 1 (100% over 33bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA760000.152No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTTGGAATTCTCGGGTGCCAA726200.14524No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG714340.14286800000000002No Hit
TGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACTC636340.127268RNA PCR Primer, Index 1 (100% over 26bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGACTTCTCGGGTGCCA632400.12647999999999998No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGTGGAATTCTCGGGTGCCA625970.125194No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA602960.120592RNA PCR Primer, Index 1 (100% over 22bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG566550.11331Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTTGGAATTCTCGGGTGCCAAG552980.110596No Hit
AGCCTCTAGTCGATAGAATAATGTAGGTGGAATTCTCGGGTGCCAAGGAA545730.109146RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)