FastQCFastQC Report
Mon 25 Sept 2023
SRR1041692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33630499
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA9753812.90028702815263No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC5479541.629336513859042No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG4793691.4253996052809088No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC3931431.1690073346815342No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA3411091.0142846824841938No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2892280.8600169744730817No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA2648350.7874845984295387No Hit
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA2534510.7536343721810372RNA PCR Primer, Index 1 (100% over 23bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA2263200.6729605766480005RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT2125880.6321285925611749RNA PCR Primer, Index 1 (100% over 25bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG1961540.5832622346757329No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC1904780.5663846974140943RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1699170.5052467404661466No Hit
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC1673100.49749484835178925RNA PCR Primer, Index 1 (100% over 24bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA1575640.4685152010382005RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC1065530.31683443055662064RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG987840.2937333757670381Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGACTTCTCGGGTGCCA918670.27316573566154934No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA797920.23726082684648836No Hit
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC781800.2324675586883204RNA PCR Primer, Index 1 (100% over 33bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG722280.21476933779662324Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG720490.2142370828336505Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGCCAAGGAA578550.17203134571390094RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA571110.1698190681024388RNA PCR Primer, Index 1 (100% over 22bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA555700.16523691783461197No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA511010.15194838470877284No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAA488630.14529371092590687No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGCATTCTCGGGTGCC487690.14501420273306084No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC487500.14495770639620897No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAAG464020.13797594855788492No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAGG449740.13372980281975594Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAGG430820.12810395706587643Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAAG426450.12680454131828373No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA411980.12250189924330292No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTTGGAATTCTCGGGTGCCAA394940.11743506987511544No Hit
CAGATCACTCTGGTTCAATGTCGGGGTGGAATTCTCGGGTGCCAAGGAAC393490.1170039136201934RNA PCR Primer, Index 1 (100% over 24bp)
AGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCAA393250.11693254982627525No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA369000.10972183314913049RNA PCR Primer, Index 1 (100% over 22bp)
CGGACGTTGACTGTCACTGATTTCTGCCCATGGAATTCTCGGGTGCCAAG351890.10463418934105022No Hit
AGGGCACCACAAGGCGTGGAGCTTGCGGCTTAATTTGTGGAATTCTCGGG349270.10385513459077726No Hit
AGCAGTGGCTGGTTGAGATTTTGGAATTCTCGGGTGCCAAGGAACTCCAG339510.10095300697143983RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)