FastQCFastQC Report
Mon 25 Sept 2023
SRR1041694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8796828
Total Bases448.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA1548341.7601117129947295No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG863710.9818425459722527No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA744810.846680189722932No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG640330.7279101057790376No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC639890.7274099254867777No Hit
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT619990.7047881349959326RNA PCR Primer, Index 1 (100% over 25bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC600520.6826551570634324RNA PCR Primer, Index 1 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA532030.6047975474796142RNA PCR Primer, Index 1 (100% over 22bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA488790.5556434660311649No Hit
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA439100.49915719620754206RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC433590.49289357482037843No Hit
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA405640.46112075852796036RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA392190.4458311564122886RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG361820.41130734851244105No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC360670.4100000591122164RNA PCR Primer, Index 1 (100% over 24bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG261490.29725487414327073No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG225050.2558308517570197Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA203240.23103782408841006RNA PCR Primer, Index 1 (100% over 23bp)
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC200500.22792306499570072RNA PCR Primer, Index 1 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA164190.18664682315034464RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG146460.166491830919054Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG125030.14213077713921426Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA121240.1378224059854302RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA119190.1354920205328557RNA PCR Primer, Index 1 (100% over 22bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC103090.11718996892970968No Hit
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA102870.11693987878357971RNA PCR Primer, Index 2 (100% over 40bp)
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA98660.11215406280536576No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA90260.10260516631676782No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA89720.10199130868535795No Hit
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT89550.10179805720880299RNA PCR Primer, Index 2 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)