FastQCFastQC Report
Mon 25 Sept 2023
SRR1041695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46262444
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC11772912.5448093490261776No Hit
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT7746331.67443164049007RNA PCR Primer, Index 1 (100% over 25bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC7296281.5771497070064002RNA PCR Primer, Index 1 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA6085371.3154017543906673RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG5879371.2708731946803329No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC4124240.8914877043677155RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2779520.6008156421653815No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA2616950.5656748268638813No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC2423340.5238244654778723No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG2412920.521572098525534No Hit
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA2359270.5099752187757309RNA PCR Primer, Index 1 (100% over 23bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA2191980.4738141374459162No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA2182160.4716914653276857RNA PCR Primer, Index 1 (100% over 23bp)
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA2074670.4484566357972787RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA1921370.41531960568274345RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA1809980.39124175973063596No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA1805570.3902885026999438RNA PCR Primer, Index 1 (100% over 23bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG1354830.2928574201570501No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1353910.2926585547447515RNA PCR Primer, Index 1 (100% over 23bp)
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC1252850.2708136215198661RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG969030.20946364182575397Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA685190.14810933897050488RNA PCR Primer, Index 5 (100% over 40bp)
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT655910.1417802310660457RNA PCR Primer, Index 1 (100% over 25bp)
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG648740.14023037779845787RNA PCR Primer, Index 5 (100% over 37bp)
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC623410.1347550942185415RNA PCR Primer, Index 1 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCCAGGAAC622350.13452596667828443RNA PCR Primer, Index 1 (95% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG561010.12126683147133342Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGAAGGTGTCTTGCTGGCGGTTGGAATTCTCGGGTGCCAAGGAACTCC527120.11394123492481287RNA PCR Primer, Index 1 (100% over 27bp)
TTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACT514390.11118954286115969RNA PCR Primer, Index 1 (100% over 25bp)
TGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACTC502670.10865617043492123RNA PCR Primer, Index 1 (100% over 26bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG494950.10698743023606794Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA491680.10628059339018059RNA PCR Primer, Index 1 (100% over 22bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA483960.10461185319132729RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTTGGAATTCTCGGGT471480.10191420064188568No Hit
TTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTC462880.10005524135300763RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)