FastQCFastQC Report
Mon 25 Sept 2023
SRR1041696.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041696.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38340984
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA8853442.309132180853783No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG6531761.7035973828945026No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA5288331.379289065716206No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG4978741.2985425726162898No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC3873361.0102401127733185No Hit
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA3067300.800005550196625RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA2841490.7411103481329535RNA PCR Primer, Index 1 (100% over 23bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA2821360.7358600916450136No Hit
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA2672020.6969096046152597RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT2489260.6492425963819812RNA PCR Primer, Index 1 (100% over 25bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC2350580.6130724240149914No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC2263590.5903839087697905RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1740730.4540128651888538Illumina Small RNA Adapter 2 (100% over 21bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG1718680.4482618390806037No Hit
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC1684500.4393470965690396RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC1623830.42352329820225787RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1597220.416582944245771No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA1257210.32790238247406484RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1227020.3200283018297079Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1168440.30474961206003476No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG1119010.29185740251215253Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA966340.25203839317217314RNA PCR Primer, Index 1 (100% over 22bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC838970.21881806684982316No Hit
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC751220.19593133029658288RNA PCR Primer, Index 1 (100% over 33bp)
CCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAGG718410.18737390777451096Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA698890.18228274996802377No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAAG695140.1813046843033554No Hit
AGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCAA680470.17747849142317265No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAAG672490.17539716768875832No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA595710.15537159922656132No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAA593960.1549151685830494No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG578610.15091161979567347Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG577360.15058559790745069No Hit
ACTGGCGAACGCCTTGTATCATCGGTGGCGTGGAATTCTCGGGTGCCAAG553220.14428946320209204No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAGG550770.14365046030117537Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGGTGTCGGGAGGCATCTCTATCTCGTGTGGAATTCTCGGGTGCCAAG548380.1430271064508934No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA542110.14139178065956784RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGTTCGTGGCGGCTAGCGTTTATGGAATTCTCGGGTGCCAAGGAACT524850.13689007042698748RNA PCR Primer, Index 1 (100% over 25bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA512430.13365071694560576RNA PCR Primer, Index 1 (100% over 22bp)
TGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAAC478050.12468381093192601RNA PCR Primer, Index 1 (100% over 24bp)
CGGACGTTGACTGTCACTGATTTCTGCCCATGGAATTCTCGGGTGCCAAG477490.12453775312600218No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTTGGAATTCTCGGGTGCCAA449020.1171122785998398No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGGTGGAATTCTCGGGTGCCA422470.11018757369398761No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA416440.10861484410520084No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCTGGAATTCTCGGGTGCCAAGGAAC411130.1072299031240304RNA PCR Primer, Index 1 (100% over 24bp)
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGTGGAATTCTCGGGTGCCAA408340.10650222226951712No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA404960.1056206590837627RNA PCR Primer, Index 1 (100% over 23bp)
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG389540.10159885307064627Illumina Small RNA Adapter 2 (100% over 21bp)
CAGATCACTCTGGTTCAATGTCGGGGTGGAATTCTCGGGTGCCAAGGAAC386620.10083726593975784RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)