FastQCFastQC Report
Mon 25 Sept 2023
SRR1041698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT16119073.223814RNA PCR Primer, Index 1 (100% over 25bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC12488752.49775RNA PCR Primer, Index 1 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA12003362.4006719999999997RNA PCR Primer, Index 1 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC7087601.4175200000000001RNA PCR Primer, Index 1 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA3312030.662406RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2735490.5470980000000001No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA2688670.537734RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC2663780.532756No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA2628580.5257160000000001RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA2552140.510428RNA PCR Primer, Index 1 (100% over 23bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA2229070.44581400000000004RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG2169210.433842No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA1803710.360742No Hit
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA1554840.310968RNA PCR Primer, Index 1 (100% over 23bp)
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT1548760.30975199999999997RNA PCR Primer, Index 1 (100% over 25bp)
AGAGAAGGTGTCTTGCTGGCGGTTGGAATTCTCGGGTGCCAAGGAACTCC1475070.295014RNA PCR Primer, Index 1 (100% over 27bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA1345780.269156No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC1253730.250746RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1172020.234404No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG1122610.224522Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG1116950.22339Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA1110260.22205200000000003RNA PCR Primer, Index 2 (100% over 40bp)
TTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACT1093060.21861200000000003RNA PCR Primer, Index 1 (100% over 25bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1063120.212624Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1031050.20620999999999998RNA PCR Primer, Index 1 (100% over 33bp)
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC1014650.20293RNA PCR Primer, Index 1 (100% over 24bp)
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT998080.199616RNA PCR Primer, Index 2 (100% over 37bp)
TTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTC942930.188586RNA PCR Primer, Index 1 (100% over 26bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG889660.177932No Hit
TGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACTC760600.15211999999999998RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGTTCGTGGCGGCTAGCGTTTATGGAATTCTCGGGTGCCAAGGAACT671640.134328RNA PCR Primer, Index 1 (100% over 25bp)
CTAATTTGCTGGTTTGAGGTTGGGTTGGAATTCTCGGGTGCCAAGGAACT671410.134282RNA PCR Primer, Index 1 (100% over 25bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA662230.132446RNA PCR Primer, Index 1 (100% over 22bp)
TGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAAC639910.127982RNA PCR Primer, Index 1 (100% over 24bp)
TGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAAC616620.123324RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA562860.11257199999999999RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACT504610.10092199999999998RNA PCR Primer, Index 1 (100% over 25bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG503500.10070000000000001Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)