FastQCFastQC Report
Mon 25 Sept 2023
SRR1041699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018290
Total Bases153.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT970863.2165895258573567RNA PCR Primer, Index 1 (100% over 25bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA709642.351132594946145RNA PCR Primer, Index 1 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC708332.3467923890679825RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC423101.4017871046188406RNA PCR Primer, Index 1 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA188640.6249896464554433RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA161350.5345742125508152RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG158610.5254962246835129No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA155650.5156893472794198RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC150770.49952125209969883No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA150310.4979972103409547RNA PCR Primer, Index 1 (100% over 23bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA135120.44767070095981504RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG122840.4069854122698614No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA104800.3472164702530241No Hit
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA90130.29861279068611696RNA PCR Primer, Index 1 (100% over 23bp)
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT89710.29722127429769835RNA PCR Primer, Index 1 (100% over 25bp)
AGAGAAGGTGTCTTGCTGGCGGTTGGAATTCTCGGGTGCCAAGGAACTCC83880.2779057015727448RNA PCR Primer, Index 1 (100% over 27bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA75990.25176507227602385No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC74100.24550324852814012RNA PCR Primer, Index 1 (100% over 24bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG68190.22592262506253544Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACT65860.2182030222410703RNA PCR Primer, Index 1 (100% over 25bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG65210.21604948497327955Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG64820.21475736261260514No Hit
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA62560.20726967918920977RNA PCR Primer, Index 2 (100% over 40bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG61800.20475169715302374Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC56910.1885504706307214RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTC56780.18811976317716322RNA PCR Primer, Index 1 (100% over 26bp)
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT56740.18798723780683763RNA PCR Primer, Index 2 (100% over 37bp)
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC54500.18056581706860508RNA PCR Primer, Index 1 (100% over 33bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG51080.16923489790576784No Hit
TGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACTC45300.15008498189372127RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGTTCGTGGCGGCTAGCGTTTATGGAATTCTCGGGTGCCAAGGAACT39710.13156456139072126RNA PCR Primer, Index 1 (100% over 25bp)
CTAATTTGCTGGTTTGAGGTTGGGTTGGAATTCTCGGGTGCCAAGGAACT38990.12917910472486077RNA PCR Primer, Index 1 (100% over 25bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA37780.12517021227251193RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAAC37530.12434192870797703RNA PCR Primer, Index 1 (100% over 24bp)
TGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAAC36930.12235404815309331RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA31950.10585463954755837RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)