FastQCFastQC Report
Mon 25 Sept 2023
SRR1041700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36658171
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT9995472.726669041944291RNA PCR Primer, Index 1 (100% over 25bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC8763752.3906675540359066RNA PCR Primer, Index 1 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA8483702.314272580593287RNA PCR Primer, Index 1 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC5144891.4034770038035995RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC3634250.9913887956930529No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG2650780.723107544017949No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA2547100.6948246272297655RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA2333850.6366520577363229RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1915060.522410133336985RNA PCR Primer, Index 1 (100% over 23bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA1340540.365686547754933RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA1158410.316003217945598RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG1154920.31505117917639697No Hit
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT1141980.31152127038743965RNA PCR Primer, Index 1 (100% over 25bp)
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG1137170.31020914818690765RNA PCR Primer, Index 5 (100% over 37bp)
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA1018490.27783437422450785RNA PCR Primer, Index 5 (100% over 40bp)
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC1004820.27410532838640533RNA PCR Primer, Index 1 (100% over 24bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA980850.2675665406220076No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA972250.2652205425087902No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG956100.2608149762845506No Hit
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA924640.2522329878378275RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC789520.21537353841248655No Hit
AGAGAAGGTGTCTTGCTGGCGGTTGGAATTCTCGGGTGCCAAGGAACTCC777310.21204276667267444RNA PCR Primer, Index 1 (100% over 27bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG699250.1907487419380525Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC697510.1902740865058434RNA PCR Primer, Index 1 (100% over 24bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG690620.1883945601104867No Hit
TTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACT669570.18265232054266975RNA PCR Primer, Index 1 (100% over 25bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG569420.1553323541428185Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTC559500.1526262725982701RNA PCR Primer, Index 1 (100% over 26bp)
CTAATTTGCTGGTTTGAGGTTGGGTTGGAATTCTCGGGTGCCAAGGAACT555720.15159512459036759RNA PCR Primer, Index 1 (100% over 25bp)
ATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA544920.1486489874249318RNA PCR Primer, Index 5 (100% over 36bp)
TGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAAC543520.1482670807553383RNA PCR Primer, Index 1 (100% over 24bp)
TGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGGAACTC458710.125131720292319RNA PCR Primer, Index 1 (100% over 26bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG436580.11909486700795847Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG386450.10541988033172742RNA PCR Primer, Index 5 (100% over 39bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA369760.100867007249216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)