FastQCFastQC Report
Tue 27 Jun 2023
SRR1042853.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042853.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35173440
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA4051851.151962958414076No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA3568201.014458636971533No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG3065680.871589472056188No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3049340.8669439213224525Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGA2630290.7478057306877007No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA2087960.5936183665856964No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1748960.4972388256593611No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1719450.48884897240645214No Hit
ATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG1719330.4888148557547968No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAG1706940.48529231147138296No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA1552730.4414495710399665No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG1366420.38848062629074664No Hit
TTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAGAG1344320.3821974762775549No Hit
TCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGG1256110.3571188942565754No Hit
TCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGAT1183410.3364498894620486No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC1061840.3018868782808847No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG1007660.2864832100584987No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAA1004560.2856018632240691No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG977460.27789718605857144No Hit
ATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAG743060.21125599315847413No Hit
TCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGA727990.20697151032142433No Hit
TTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAAG715460.20340916327774594No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG696010.19787942265527625No Hit
TCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATC694300.19739326036918767No Hit
TTAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAG691100.19648348299171192No Hit
AAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGA648230.18429530918784173No Hit
TTCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG644360.18319504717195703No Hit
TTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAAGA618880.1759509448038065No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT613800.17450667321706378No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT603610.1716096008806645No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG586280.1666825877707725No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGA572390.16273358534166688No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC563160.16010944621851034No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA530660.15086951972852244No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT528170.15016159920667413No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC511460.14541085546366803Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG500480.14228918183720443No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG499350.14196791670078332No Hit
AAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGA493270.14023933968357943No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG489700.13922436929683304No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA485320.13797911151141315No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA469220.13340179408098837No Hit
AATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAA465940.13246927226907576No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA465870.13244937088894349No Hit
TAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGA464690.13211389048099928No Hit
ATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAGAG457380.13003561778432818No Hit
TAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCAATAGATCGGAA445960.12678884976846166No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA441030.1253872239962881No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG430300.12233662672743979No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT427840.12163723536850533No Hit
ATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCAATAGATCGG420850.11964994040958177No Hit
ATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGA415710.11818861049701138No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC413850.11765980239635361No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT413180.11746931775794463No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGAT405720.11534839924670433No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCG390240.11094735118316548No Hit
ATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGC377340.10727981113021644No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC376740.10710922787193974No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT373390.10615680467989483No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA370750.10540623834347734No Hit
TCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAG364730.10369471965210114No Hit
TGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAGA363390.10331375037528318No Hit
TCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCAATAGATC357640.10167899415013146No Hit
TGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCG356790.10143733453423948No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA353580.10052471410245913No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT352000.10007551152233049No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)