FastQCFastQC Report
Tue 27 Jun 2023
SRR1042854.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042854.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29710852
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGA2550850.858558347636749No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG2048940.6896268070669935No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA1803400.6069836031629117No Hit
ATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG1454670.48960898193023883No Hit
TCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGG1053760.3546717542802206No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG1029780.3466006292919503No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG968800.3260761421449644No Hit
TCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGAT965900.3251000678136056No Hit
ATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAG915800.3082375422959934No Hit
TCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATC913280.3073893673597782No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA908980.30594208473052203No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAG908160.30566609129889644No Hit
ATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGC798130.26863248485772134No Hit
TTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAGAG765510.25765333151671316No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGAT760700.25603439443608017No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC750740.25268208397389613No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG750280.2525272583903013No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA713090.2400099465340139No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA663530.2233291727884478Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG613520.20649693923284326No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC602540.2028013198679055No Hit
TTAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAG601750.20253542375694913No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA591040.19893068027803446No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAA587840.19785363273998338No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT527840.1776589914015256No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC495480.16676734817298405No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT493720.16617497202705597No Hit
TTCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG488460.1644045751363845No Hit
TTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAAG483900.1628697823946617No Hit
TAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCAATAGATCGGAA449440.15127132671927415No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC447650.15066885325267682No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA436000.14674772705945963No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG427700.14395413500763965No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA426710.14362092342555507No Hit
TAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGA424800.14297806067628085No Hit
TCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGA394620.1328201560830366No Hit
ATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGA388950.13091176247655234No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG384590.12944428520595774No Hit
TTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAAGA361350.12162222746086178No Hit
AAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGA356720.12006387430424412No Hit
ATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCAATAGATCGG351650.11835742711114444No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT346190.11651971474934478No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA326600.10992616435233833No Hit
TCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCAATAGATC323580.10890970073830263No Hit
ATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAGAG322380.10850580791153348No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG318940.10734798180812857No Hit
TTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGAT317640.10691043124579531No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG317310.1067993607184338No Hit
TCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAG315640.10623727653451338No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA303180.10204352268322699No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCG302680.10187523400540652No Hit
AAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGA301420.10145114653729889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)