FastQCFastQC Report
Thu 29 Jun 2023
SRR1042855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48132696
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA11777432.446866886492292No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA9592251.9928761106587505No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG7023771.459251316402472No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT5502011.1430920054841724No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA5395101.120880492545026No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA4086720.849052793552225No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3712880.7713841751145625Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT3527510.7328718923203471No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3103450.6447696177251322No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG2500780.5195595110649941No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAG1974690.41025958737071366No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGA1684980.3500697322252633No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1479930.3074687526333451No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1439410.2990503586169368No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG1363240.2832253568343647No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1327120.27572110234589814No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1157630.24050803221161765No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA1125310.2337932618609188No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT1086110.22564910970289304No Hit
TAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGA1053490.21887201165710726No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACCTGTAGGCACCATCAATAG1033900.2148020131679306No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1016620.2112119379309233No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCG965470.20058506591860134No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG944240.1961743426962828No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCCTGTAGGCACCATCAATAGATCG938940.19507322008308034No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC918670.19086194548503993No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC905740.18817562182679315No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG881960.18323511319623567No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT877240.1822544907935346No Hit
TTAAGGCAAAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCG876170.1820321886810579No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG875150.1818202745177623No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA863400.1793791064601908No Hit
TGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAGA855750.17778975023547403No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA851170.1768382140904802No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA845660.17569346209071687No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG826690.17175227417138655No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA818450.17004034014633213No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA806840.16762825834646786No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCAA776510.161326928373179No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA759550.15780333601093113No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG743020.15436908001164115No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA718130.14919795890926202No Hit
ATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG717910.1491522519328649No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC699190.14526300375944037No Hit
ACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAATAG690710.14350120757831641No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGAT679780.14123040188731587No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA637850.13251906770399896No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGA622480.12932581212571181No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA605160.1257274265293596No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG587300.12201685108185088No Hit
TCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGA585550.12165327286051046No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAA574570.11937207922032872No Hit
ATAAATCATTTGTATACGACTTAGATGTCTGTAGGCACCATCAATAGATC564460.11727163589589913No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCATCATCAATA544630.1131517752506529No Hit
TCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGAT538150.11180549703677517No Hit
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAAGAG523030.10866418120439379No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCATCATCAATAGA522780.108612241458488No Hit
AAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA508580.10566206389103988No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC506930.10531926156806178No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT497700.10340164614922047No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT490920.10199304024025582No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT487750.10133444426217056No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA487390.10125965102806625No Hit
TCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATC487050.10118901297363439No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT482810.10030811488307241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)