FastQCFastQC Report
Tue 27 Jun 2023
SRR1042856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39447031
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA5857501.4849026280330198No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG4378271.109911161628362No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG3512790.8905080841191826No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA3446390.8736753851005922No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1938680.4914641104421775No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC1785940.452743832609354No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA1493070.3784999687302195No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1473260.37347804452000455No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGAT1394150.35342330326457266No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG1250410.3169845659613774No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1238510.31396786237220237No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG1107190.280677650999894No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCG1065550.2701217234828142No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA1022050.25909427758961123No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA945510.23969104290763985No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA939760.23823339201371074No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG921910.23370833662994814No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG829060.21017044349928388No Hit
TAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGA824390.20898657746891014No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA820350.20796241927561035No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA797330.20212674560982805No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA779470.19759915518103252No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA771040.19546211221828075No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT748220.1896771394531568No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG713220.18080448183793604No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC662620.16797715397135973No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT654430.16590095208939806No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC643880.16322647957966724No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG643590.16315296327371254No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA643210.16305663156246156No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT642270.16281833732936707No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGG573090.14528089579162495No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAG568010.1439930929149015No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAA565800.14343284796262615No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA560550.14210194932034304No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT533650.13528267818178763No Hit
ATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG531650.13477566917520356No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG526960.13358673305476398No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC520320.13190346315290497No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA514570.13044581225897584Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT514010.1303038497371323No Hit
TGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAGA508860.12899830154517838No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG500280.12682323290693284No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT471160.11944118177106915No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGA461580.11701260862953158No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA449380.11391985368936891No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA445680.11298188702718844No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT437560.11092343046045722No Hit
ATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGC426280.10806389966332319No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG424930.10772166858387898No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG403420.10226878671806758No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)