FastQCFastQC Report
Tue 27 Jun 2023
SRR1042857.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042857.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27983305
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG344577012.313663450403732No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG9072153.2419866059423645No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC8033722.870897486912286No Hit
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4602591.644762832696138No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT3246591.1601881907801812No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1985640.7095802300693217No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG1728040.6175253423425145No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATAGA1494860.5341970864413621No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA1464400.5233120247947839No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC1416790.506298308938133No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1383450.49438406221137926No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1317170.47069851113011846Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG1220090.43600639738586994No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA1091410.39002183623414033No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC858680.3068543905017652No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA835830.2986888074871785No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT834340.29815634715055994No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA817330.2920777227707735No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATAGA813050.29054823938773494No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG811280.2899157193905438No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA748630.26752737033742086No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG733910.2622670910387461No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC721950.25799311410857295No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG674450.2410187074042898No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG617930.22082095020584594No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT610900.21830873801361203No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCCTGTAGGCACCATCAATAGAT539560.19281496592343184No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG534830.19112467237161584No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA534720.19108536321924804No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG521670.1864218683247029No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG505390.18060411377426647No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATAG436250.15589652473144255No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG423640.15139026644636866No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGAT420690.15033606645105No Hit
ATGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG382580.13671723193525567No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATAGAT378470.1352484990604219No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA374810.13394057635436557No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATA370240.13230745975144823No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG361230.12908768281659366No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT327290.1169590225314701No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC312950.11183453848642969No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC288640.10314721581314289No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)