FastQCFastQC Report
Tue 27 Jun 2023
SRR1042858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26140273
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG273748010.472270125105426No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG10339293.955310642700633No Hit
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3683771.409231648039789No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2660071.0176137028102192Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1631470.6241212553518474No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1294970.49539268392491537No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACCTGTAGGCACCATCAATAGATCGG1034980.39593312587056756No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATAGA1019480.39000357800394814No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG1008960.3859791364841522No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG899030.3439252528081861No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG881370.33716939375499255No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC753590.28828696624553235No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG744910.28496641944022544No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC707160.2705251012489426No Hit
TATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGA650660.24891094289642654No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA569940.2180313878129735No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATAGA569760.21796252854742565No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT462720.17701421863497754No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA462390.17688797664813982No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCCTGTAGGCACCATCAATAGATCG458540.1754151534683666No Hit
TCGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAA448210.1714633967288712No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA446880.1709546032667677No Hit
TTGGGCCAGCATCAGTTTTGGTGGCCTGTAGGCACCATCAATAGATCGGA441730.168984463169149No Hit
AAGAGGTGCACAATCGACCGATCTGTAGGCACCATCAATAGATCGGAAGA426830.163284446187689No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT413950.15835718318626588No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA396310.15160897516257768No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA396050.15150951177900857No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA389580.14903440373403906No Hit
AGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCGGAAG385830.1475998357017924No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA380540.14557613839763647No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG368640.1410237758419738No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA366970.14038491487827998No Hit
ACGTGAAATTGTTGAAAGGGAAGGGCCTGTAGGCACCATCAATAGATCGG362500.13867490978384198No Hit
AGTTGGTGGAGTGATTTGTCTGCTTACTGTAGGCACCATCAATAGATCGG358760.137244167266348No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG350780.13419140649372713No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA347690.1330093224351559No Hit
AGCTGGGACTGAGGACTGCGACGTCTGTAGGCACCATCAATAGATCGGAA333440.12755796391261867No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG327350.12522822542825013No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGA325890.12466970027436208No Hit
ATGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG325580.12455110931702972No Hit
AGTTGGTGGAGTGATTTGTCTGCTCTGTAGGCACCATCAATAGATCGGAA320190.1224891568653472No Hit
TAGTTGGTGGAGTGATTTGTCTGCTCTGTAGGCACCATCAATAGATCGGA319450.12220606877365052No Hit
AGAATCCATGCTAGAACGCGGTGATTCTGTAGGCACCATCAATAGATCGG312430.11952055741728482No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA311550.11918391211905094No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT308300.1179406198244372No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTCTGTAGGCACCATCAATAGA304160.11635685671683689No Hit
TGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATAGAT301580.1153698739106512No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG297590.11384349352434077No Hit
AAATGTAGATAAGGGAAGTCGGCAAAATCTGTAGGCACCATCAATAGATC292040.11172033283661574No Hit
AAATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGA292010.11170885629235777No Hit
AGTTGGTGGAGTGATTTGTCTGCTTCTGTAGGCACCATCAATAGATCGGA287700.11006005943396231No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG284840.1089659622147022No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG284510.10883972022786448No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC281220.10758112587424011No Hit
AGTTTTCATGCCAGGTCGTACTGATAACCGCCTGTAGGCACCATCAATAG277680.10622689365179928No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT275740.10548474378978367No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC271530.10387420207891478No Hit
TAGTTGGTGGAGTGATTTGTCTGCTTACTGTAGGCACCATCAATAGATCG269910.10325446868898423No Hit
TGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGGA268070.10255057397449523No Hit
NGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG263210.10069137380470357No Hit
AGTGAAACTGCGAATGGCTCATTAAATCCTGTAGGCACCATCAATAGATC262950.10059191042113447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)