FastQCFastQC Report
Tue 27 Jun 2023
SRR1042859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26831836
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA5764772.148481378613077No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG4455431.6605013536904443No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2691251.003006279555376No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1594390.5942157666735888No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA1251060.46625955823522475No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG1221420.4552129790894667No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1193730.444893148571719No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG1127940.4203737679374606No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1108630.41317709306213707No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1070720.39904835435040675No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA1004930.37452897371614824No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA976360.36388117458678565No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA899500.33523609789505276No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT860530.32071230608296797No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT849080.31644498721593256No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG737480.27485260419749136No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG626470.23348010922547383No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGAT616170.22964138570316248No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA516250.19240204062070146No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC490850.1829356738763609No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT474450.17682353156899142No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG473870.17660737043860883No Hit
AAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGA459520.17125924591966052No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT447820.1668987541515981No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA427220.15922130710697546No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC421530.15710069187960155No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG421080.15693298065775296No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG410770.15309053021940056No Hit
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG395620.14744425241716594No Hit
AATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAA388670.14485404576861605No Hit
TTAAGGCAAAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCG381700.14225638528798404No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT374890.13971835546400926No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC373380.1391555911418063No Hit
TTGGGCCAGCATCAGTTTTGGTGGCCTGTAGGCACCATCAATAGATCGGA367630.1370126144181859No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG352510.13137751736407452No Hit
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAAGAG350480.1306209534077355No Hit
AAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA344790.12850033818036158No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT336790.1255188053474984No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA328320.12236210746070451No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG324440.12091606403676587No Hit
AAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGA324280.12085643338010862No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG314160.11708479434653671No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA307560.11462502975942458No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA305670.11392064262766068No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT298730.11133416289515186No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC293780.10948933945481777No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA287260.10705939019603429No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT274150.10217340326617978No Hit
TTAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAG271570.10121185892758139No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA268370.10001924579443612No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)