FastQCFastQC Report
Tue 27 Jun 2023
SRR1042860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28232662
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG26787389.488081570204042No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC10573803.74523663408006No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT8200262.9045295126616115No Hit
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3570461.2646558089350555No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3485631.2346090496177795No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC2333020.82635495016375No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT2170260.7687054093588483No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATA2134720.7561171525377238No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG1713630.6069672069888415No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA1495060.5295497817386119No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1444780.5117406215538584No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG1434990.5082730066332392No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATAGA1386000.4909207640427247No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1130420.4003944084337495Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC1046310.37060267289000237No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT975900.3456634730370094No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT878760.31125651559176387No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA874120.30961302905124566No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG838440.29697518427415737No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA807260.28593123808162335No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG806350.2856089163678579No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC754330.26718344873041017No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATAGA735140.26038635676649974No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT724340.2565610001635694No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG715400.2533944549755882No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA655950.2323372836751986No Hit
TGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAATAGA640830.22698178443109612No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC632520.22403838504495255No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG624530.22120832955815503No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG616250.2182755561625751No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAAT590200.20904865435643297No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATC574380.203445215332511No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT573870.20326457349292815No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG564860.200073234326965No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCCTGTAGGCACCATCAATAGAT539220.19099155439186002No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG496030.17569366997699332No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC467440.16556710096979166No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC458530.16241118177237412No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT432230.15309573004486787No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG416250.14743561907127284No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCG401380.1421686697485345No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATAGATC391380.1386266728939694No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCCTGTAGGCACCATCAATAG382740.13556638761162515No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGA376790.13345889948315892No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA376270.1332747156467215No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG376060.13320033371277565No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAATA362650.12845051593080384No Hit
AGAAGTCAGCAACTTGATTCCAGCAGTAGTCTGTAGGCACCATCAATAGA343840.12178801984736684No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATAG341410.12092731461170753No Hit
AAGAGGTGCACAATCGACCGATCCTGATCTGTAGGCACCATCAATAGATC340060.12044914503634124No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG329920.11685756022581222No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG321890.11401333675159643No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA320180.1134076552894658No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA319020.11299678365433626No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG317960.11262133198775234No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATAG315420.11172166478669279No Hit
ATGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG310710.11005338426819262No Hit
CCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT310160.10985857444119156No Hit
NTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC307370.10887035731876789No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGAT295780.10476518296432691No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA289200.10243454903402308No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATAGA283010.10024205298104728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)