FastQCFastQC Report
Tue 27 Jun 2023
SRR1042861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27962922
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG27480489.827470820109573No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG10108673.615026355257151No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7975692.8522376881786533Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3643221.302875286066313No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC3293541.1778239770507533No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA2254160.8061246246011057No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC1767850.6322121844061933No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1321080.4724398973755318No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1220080.4363206391663933No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA1054060.37694916146459945No Hit
CAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1046590.37427776682279484No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1012270.362004371360046No Hit
TAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA989920.3540116444197069No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG984090.3519267407032784No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA886820.3171413917329527No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC859870.3075036292702172No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATAGA780210.27901590541932636No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG778770.2785009377775327No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC774990.27714914771782434No Hit
TGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAG658270.23540815941910506No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA618550.22120363529963No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG616560.2204919786279846No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG615590.22014509070260968No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC609300.21789568343394153No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG543300.19429299985173223No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA532210.19032703377708524No Hit
AATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAA521540.1865112665979614No Hit
ATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCGGAA485470.17361204240386607No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATAGA482240.1724569413740095No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG469650.1679545506724941No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA426160.15240181265748978No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA409180.1463294858813396No Hit
AAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGA397500.14215252612012436No Hit
ATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAG393370.1406755703141467No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG383060.13698854504547128No Hit
GTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGA373130.13343741401560252No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG364570.13037621747827355No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT358000.12802667761258998No Hit
TCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATC357410.12781568392602174No Hit
TAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGA352620.12610270128422202No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA351960.12586667444839994No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA351230.12560561446332397No Hit
AGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAG341860.12225474862748607No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC337660.12075275967225456No Hit
ATGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG332420.1188788496423943No Hit
CACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCA325370.11635765389611287No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG309310.11061433422444192No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGA302620.10822188038860887No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT298000.10656969253785423No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATA296080.1058830690154627No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA292950.10476372962739731No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG290490.10388399323933313No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA289670.10359074777664509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)