FastQCFastQC Report
Wed 28 Jun 2023
SRR1042862.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042862.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28318151
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA11537084.074093679350746No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA6027632.1285393951038682No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4474591.5801137581334317No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3062241.0813700371892219No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC1197860.4230007813716369No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1089470.3847249772769416Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1038520.3667329833787524No Hit
TGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATCG937050.33090084165452754No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG893430.31549729359095513No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG806000.2846231026877426No Hit
NTGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA773360.27309692642009004No Hit
TTTCTTAGTTGGTGGAGTGATTTGTCTGCTTACTGTAGGCACCATCAATA683130.24123397039587788No Hit
NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG595620.21033152906063676No Hit
ACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGAGCA592120.20909557265938725No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA552520.19511160880525003No Hit
TCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGA526790.18602556360406441No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG520790.1839067812019224No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC509260.17983518768580617No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA488570.17252891970241985No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA487820.1722640719021521No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG479460.16931190175516758No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA474300.16748974888932544No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG467810.1651979325910085No Hit
TGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCG461730.16305089975683792No Hit
TACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAG441270.1558258517655337No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG424230.14980850974345042No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG421980.14901396634264716No Hit
TCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATC406420.14351925731309223No Hit
TACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGA398470.14071187063025406No Hit
NTGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA394890.139447663796976No Hit
TTTCTTAGTTGGTGGAGTGATTTGTCTGCTTCTGTAGGCACCATCAATAG393740.13904156383656546No Hit
TGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAA375250.13251218273396453No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG367500.12977542213119775No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA363960.12852534051393397No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT363470.12835230661775904No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACTGTAGGCACCATCAA360630.12734941628074514No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGCTGTAGGCACCATC357630.12629002507967416No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC354290.1251105695424818No Hit
TAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGA352760.12457028002993556No Hit
ACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGA345750.122094835923433No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA345550.1220242098433616No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA343910.1214450759867761No Hit
ATGTACACGGAGTCGACCCGCAACGCGCTGTAGGCACCATCAATAGATCG341370.12054812476986934No Hit
ACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGAG339690.11995486569726958No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA325080.11479563054805379No Hit
TTCTTAGTTGGTGGAGTGATTTGTCTGCTTACTGTAGGCACCATCAATAG324090.11444603145170035No Hit
GAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGA309990.10946689280666665No Hit
AGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCA301920.10661713047578567No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG296820.10481616543396495No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA293940.1037991498809368No Hit
TGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAA289610.10227009524739097No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC289380.10218887525530887No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG288550.10189577702301256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)