FastQCFastQC Report
Tue 27 Jun 2023
SRR1042863.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042863.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32402573
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG16480585.0861948524890295No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG9153262.824855914991689No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA6290451.9413427446024119No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA4743681.463982505339931No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA3297941.0178018887574145No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA3078420.9500541824255746No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2428620.7495145524400176No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2415300.7454037677810339No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2051620.6331657674222353No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1966060.6067604569550695No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA1964530.6062882722307269No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1756140.5419754783053803No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1601560.4942693902734206No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1475750.4554422267639055No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1407880.43449635928603575No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG1317160.4064985826897142No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1295890.3999342891689496No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1229040.3793032115073084No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG1218030.3759053331968421No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA1081880.33388706507967747No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1043480.3220361543510758No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1018330.31427442505877545Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1013700.3128455261870716No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA972470.3001212280271693No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG948980.2928718037299075No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG922630.2847397334773384No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC894610.2760922720550618No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA890720.2748917501088571No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA866750.26749418942748776No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC866560.2674355521087785No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG865280.26704052175115844No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA850780.26256556848124374No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA842660.26005959465009154No Hit
TCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGA834160.25743634618152084No Hit
ATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC833320.25717710750933265No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG801720.2474247955555875No Hit
TTGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG801040.24721493567810188No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG709770.21904741947499046No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA705520.21773579524070513No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT699360.2158347116446586No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG690200.21300777564794005No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC667640.20604536559488654No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC653450.20166608373970796No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA636620.196472051771938No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG624520.1927377804225609No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC614790.18973493246971468No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG588620.18165841336118585No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG571950.1765137601881184No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG561300.17322698416573276No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG555730.17150798487515173No Hit
TTCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGG543240.16765335271368728No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG537670.16593435342310622No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT525430.16215687562836445No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA521440.16092549193547068No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT519890.16044713486179013No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA514710.15884849638329646No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT502530.1550895356365681No Hit
ATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGC490400.1513460057631843No Hit
TACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG467710.14434347543943502No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA453310.13989938391620937No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA451580.1393654756984885No Hit
ACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGAAG435270.1343319248135017No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG434390.1340603414426379No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT425070.13118402665121687No Hit
TAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA420190.1296779734127904No Hit
TGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA419140.1293539250725552No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC418750.12923356426046784No Hit
TTCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA413340.12756394376458932No Hit
TGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA390180.12041636323140141No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG351700.10854076310544845No Hit
TGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAG351170.10837719584799639No Hit
TCAATATAGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAG350650.10821671476521325No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA344400.10628785559714657No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA337780.10424480796633034No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA335980.10368929652592712No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA328330.10132837290421351No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG325790.10054448453831119No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG324980.10029450439012977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)