FastQCFastQC Report
Tue 27 Jun 2023
SRR1042864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68689532
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA18773172.7330467180938136No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA14651842.133052820916002No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG11227951.6345940455672343No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA11117331.6184896994202842No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA10659341.5518143288558146No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC9524411.386588279564927No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA9480401.3801811897626555No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG7825631.1392754866927903No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA7694241.1201473901438141No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6566500.9559680796777011No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA6056580.8817326051952137No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC5072920.7385288343498978No Hit
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAAGAG4323000.6293535381781317No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG4214920.6136189718107266No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA3903720.568313669686962No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA3002710.4371422999358912No Hit
TGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA2991940.43557437543758487No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2891330.42092731102025854No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2657470.3868813664358639No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT2637700.38400319862420956No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT2621020.3815748810168047No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG2448250.35642257687823525No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT2343190.34112766993375354No Hit
TGATGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2074240.30197323225320566No Hit
GAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGA2065800.30074451519046597No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC2000170.29118992978435204No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGA1981730.28850538681789245No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCG1978840.2880846531317174No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA1882450.2740519472457608No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1807540.26314635540099474No Hit
AGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1787770.2602681875893404No Hit
GAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA1755370.2555513116612878No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC1667640.2427793510079527No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1654550.2408736748999833No Hit
TGGTCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA1628000.23700845712560686No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG1610590.23447386422723043No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA1597520.2325710997710685No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGG1494480.21757026965913817No Hit
TGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA1449530.211026332221917No Hit
AAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1435350.20896197109044212Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG1401330.20400925136598688No Hit
TTAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA1380780.2010175291338424No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA1309930.1907030026059866No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC1309930.1907030026059866No Hit
GAAAAGAAACCAACCGGGATTGCCCTGTAGGCACCATCAATAGATCGGAA1300500.1893301587787787No Hit
AAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA1281830.18661213181653355No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT1277760.18601961067371955No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1240650.18061704074501483No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT1207110.17573420066393813No Hit
TGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA1185560.17259689584142165No Hit
TTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1166540.16982791497254632No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGA1165260.16964156925687018No Hit
AAAAAGAAACCAACCGGGATTGCCCTGTAGGCACCATCAATAGATCGGAA1163990.16945667936709774No Hit
TATAGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1160600.16896315438573667No Hit
AATACTGCCAGCTGGGACTGAGGCTGTAGGCACCATCAATAGATCGGAAG1149340.16732389441814802No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA1132690.16489994428845434No Hit
TACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAAGA1026930.14950312953071218No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG1024380.1491318939252636No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA994960.14484885411651952No Hit
CGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA993090.1445766146725239No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA988680.1439345954489834No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC922350.13427810222960904No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG921980.13422423667117137No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA921240.1341165055542961No Hit
TAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG896920.1305759369564492No Hit
GAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT882850.12852758990991522Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT869090.1265243734663966No Hit
AAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGAA805690.11729443723681215No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG794990.11573670351983181No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG791030.11516019646195873No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG771610.1123329825569346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG765310.11141581223759103No Hit
CTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG747820.10886957273198485No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGAT745910.10859150998437433No Hit
ATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC729800.10624617445348152No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA726690.1057934125974246No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC715740.10419928323285126No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC706600.10286865835685123No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG696430.10138808341276806No Hit
CGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT689220.10033843293618595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)