FastQCFastQC Report
Wed 28 Jun 2023
SRR1042865.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042865.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79026619
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA28064083.551218609010718No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA14788321.8713087042228138No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA13958231.7662694136010046No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA12178001.5409997484518476No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG9699911.2274231294141533No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA9168701.1602040067031085No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7820510.989604528064145Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG7730010.9781526905510155No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA7679220.9717257421831497No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG6628010.8387060061369954No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA5383270.6811970533624879No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA5256710.6651821963938505No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG5230370.6618491422491453No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA4913420.6217424030249858No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC4345910.549929891344586No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3606370.45634876521795775No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3504210.4434214754904293No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA3276680.4146299109670882No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA3170920.4012470785318552No Hit
TCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATC2597170.3286449594914342No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT2566770.32479815440415083No Hit
TGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATCG2545170.3220648981579232No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG2521520.3190722356475861No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG2478460.31362343870487486No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG2343860.2965912030223639No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG2336410.2956484826966974No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2152840.27241960079299354No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC2057180.260314818732154No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2007450.2540220023837791No Hit
TGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA1865560.23606729271816623No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1757350.2223744381624121No Hit
TGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA1725060.21828847315358385No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1686040.21335089636063007No Hit
TCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGA1660470.2101152777395171No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG1595670.20191550900083427No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1580200.19995794075411474No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1521840.19257308730365905No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT1421860.179921653993574No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1337980.16930750890405674No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA1291300.1634006384608204No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGG1255340.15885027296941553No Hit
ATGGACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA1235060.15628404904934626No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC1229650.15559946959137907No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG1227780.15536284046265475No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA1226960.1552590779570109No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT1191170.150730224204581No Hit
TGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCG1173700.14851957667580337No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG1137890.14398819213055286No Hit
TACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAG1091430.13810916040834292No Hit
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAAGAG1090340.13797123219962124No Hit
TTAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA1076720.1362477622888055No Hit
TGATGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1073330.135818792905717No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA1061860.13436738322311373No Hit
TGGTCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA1058060.1338865325872033No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG1017180.12871359206193547No Hit
TGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAA999640.12649408675828583No Hit
TTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG997420.12621316875520133No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA991930.12551846612595183No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGCTGTAGGCACCATC983410.12444034838438427No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT981600.12421131163412166No Hit
AATACTGCCAGCTGGGACTGAGGCTGTAGGCACCATCAATAGATCGGAAG977600.12370515307000543No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG975880.12348750488743546No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA955150.12086433812890313No Hit
AAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC932640.11801593080933907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA913410.11558257351235032No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA893770.11309733496253965No Hit
AAAAAGAAACCAACCGGGATTGCCCTGTAGGCACCATCAATAGATCGGAA891910.1128619712302256No Hit
TTGTGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG887700.11232923934149328No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG880920.11147130057531628No Hit
GAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA873860.11057793070965113No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC873240.11049947613221311No Hit
AAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA873150.11048808756452051No Hit
TAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGA852820.1079155366623998No Hit
AGATGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA848320.10734610827776905No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG841250.10645147301569362No Hit
AGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCA828540.10484315417821431No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC822080.10402570809716659No Hit
GAAAAGAAACCAACCGGGATTGCCCTGTAGGCACCATCAATAGATCGGAA808090.10225541851917011No Hit
AAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGAA807620.10219594488788644No Hit
ACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGA800170.10125322456221997No Hit
TATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGG790560.10003717861193076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)