FastQCFastQC Report
Sun 2 Jul 2023
SRR1042866.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042866.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159138350
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA69275134.353138636915615No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA35836792.2519267040282873No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA31693511.991569599659667No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA23952361.5051280850907403No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA20872171.3115738601034885No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG19201311.2065796836526204No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG18144691.1401833687480107No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA18024151.132608827476218Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA15261070.9589812889225005No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG13290340.835143760130729No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA12055270.7575339319529202No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA11382480.7152568818264108No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA11021340.6925634204451662No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG10889610.6842857174276346No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC10678930.6710469223791751No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA8595840.5401488704639705No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC8139390.5114662807550788No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA7192190.4519457440648342No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA6767500.42525890208111367No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT6024640.37857876495514753No Hit
TGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATCG5707130.35862694315983545No Hit
TCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATC5662500.3558224651694579No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG5625240.3534811062198395No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG5622610.35331584121614934No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG5444060.34209604410250577No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG5421730.3406928625312503No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA4868670.3059394545689333No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC4844130.30439740012385447No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4542100.28541831682935004No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT3966190.24922905132546616No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA3799600.23876080152898405No Hit
TCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGA3738630.2349295440099762No Hit
TGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA3667710.23047304436674126No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG3628740.22802423174552205No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT3616620.2272626302836494No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3469320.21800653330891015No Hit
TGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA3396230.21341367432802966No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT3216580.20212475496949664No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGG3074400.19319039062551546No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT2941340.18482911253007211No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG2879280.1809293611502193No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC2760810.17348489537562756No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA2664630.16744109763611348No Hit
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAAGAG2625760.16499856885533876No Hit
TTAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA2531150.15905342741080325No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT2469200.15516058825543938No Hit
TGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCG2445210.15365309493280532No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA2437930.1531956313484462No Hit
TACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAG2433130.15289400700711048No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGCTGTAGGCACCATC2390950.150243483107623No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG2355340.1480058075253388No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA2352940.14785499535467095No Hit
AATACTGCCAGCTGGGACTGAGGCTGTAGGCACCATCAATAGATCGGAAG2220270.1395182242369611No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG2211380.1389595908214456No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT2203690.13847636349126405No Hit
TGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAA2171260.13643851403511473No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA2113290.1327957717294417No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA2088080.13121161555338484No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA2087550.13117831119902904No Hit
TTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2059710.12942889001928196No Hit
AAAAAGAAACCAACCGGGATTGCCCTGTAGGCACCATCAATAGATCGGAA2029770.12754750819020055No Hit
AAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA1983640.12464877259315559No Hit
TATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGG1925570.12099974644703805No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1911720.12012943454547569No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC1903360.119604105484316No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1891410.118853186551199No Hit
TAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGA1866060.11726023299851984No Hit
AAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGAA1854810.1165533009485143No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1846730.11604556664059919No Hit
ACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGA1831750.11510424734201405No Hit
AACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA1805680.11346605013813453No Hit
AGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCA1802820.113286332301422No Hit
GAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA1786940.11228845843883639No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC1781710.11195981358358938No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1735710.10906924697912225No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA1728120.10859230348938519No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1720840.10813483990502602No Hit
TGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAG1709100.10739711703684246No Hit
AAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1685320.10590281977914184Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGA1678130.10545101165118276No Hit
AGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1677760.10542776144153813No Hit
GAAAAGAAACCAACCGGGATTGCCCTGTAGGCACCATCAATAGATCGGAA1656430.1040874182747276No Hit
TTGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA1654090.10394037640832647No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG1651860.10380024676641425No Hit
TGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAAT1627800.10228835475546906No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGA1603610.10076829375194603No Hit
TACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGA1603470.10075949637532372No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1594720.10020966033643054No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)