FastQCFastQC Report
Wed 28 Jun 2023
SRR1042867.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042867.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140917645
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA58387924.143407307154473No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA30479412.162923599808952No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA27512161.952357350280726No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA21228681.5064600320279267No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA17400761.2348176837613203No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG16928161.201280364854238No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA15210811.0794113114791266Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG15005141.06481626200892No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA13550570.9615949798195961No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG11750980.8338898936325541No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA10278260.7293806251161805No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA10166520.721451171001332No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG9793460.6949775523143322No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA9689630.6876094189624018No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC8996470.6384204050528945No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA7081660.5025389120006938No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6947160.4929943301280688No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA6227640.4419347200983951No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA5976600.4241200596277351No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT5193680.36856136788263816No Hit
TCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATC4949620.35124203218127864No Hit
TGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATCG4924080.34942962607698985No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG4844940.34381357991045053No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG4819740.3420253013737208No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG4649110.3299168106307766No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG4628690.32846773730855355No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA4215570.29915132345562545No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC4033120.2862040449228342No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3892510.27622587646848623No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT3345880.23743513454259046No Hit
TGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA3241150.2300031341000625No Hit
TCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGA3230380.2292388579159125No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG3114520.2210170344529956No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA3109900.22068918338792845No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3105380.22036842866626108No Hit
TGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA2995760.21258941703148673No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT2973990.21104454307336742No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2640660.18739030161907685No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT2585880.18350292470470964No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGG2491600.1768124921474525No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG2456490.1743209659798104No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC2371320.1682770103062679No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA2277900.1616476063022484No Hit
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAAGAG2195860.15582576617711716No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA2167520.15381466245763614No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT2140680.151910003889151No Hit
TGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCG2135090.15151331829310657No Hit
TTAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA2106440.14948021590908647No Hit
TACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAG2098320.1489039928250291No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG2071240.14698230303238463No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGCTGTAGGCACCATC2049120.1454125918723663No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG1947780.13822115747108887No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA1941110.13774783136632748No Hit
AATACTGCCAGCTGGGACTGAGGCTGTAGGCACCATCAATAGATCGGAAG1908900.13546209915727728No Hit
TGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAA1887980.13397754411805562No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA1871970.13284141953976025No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1828150.12973180186200245No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA1792640.12721189032076147No Hit
TTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1768450.1254952848523689No Hit
AAAAAGAAACCAACCGGGATTGCCCTGTAGGCACCATCAATAGATCGGAA1768100.12547044765046989No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA1741140.12355727346990505No Hit
AAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA1738380.12336141439207277No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1656760.11756937890922035No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1647750.11692999836890547No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC1633650.11592941394954479No Hit
TAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGA1601510.1136486491808744No Hit
AAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGAA1586430.11257852059619645No Hit
ACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGA1580370.11214848218617335No Hit
GAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA1567620.11124369840270891No Hit
TATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGG1566000.11112873763963341No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1564430.11101732504825779No Hit
AGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCA1562830.11090378355386227No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC1535740.1089813841268778No Hit
AAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1535730.10898067449253782Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG1517710.10770191341190807No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA1500540.10648347125017593No Hit
AACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA1488640.10563900638560914No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1479740.10500743182303394No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA1465420.10399123544819386No Hit
TGAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGA1459520.10357255118761033No Hit
GAAAAGAAACCAACCGGGATTGCCCTGTAGGCACCATCAATAGATCGGAA1449450.1028579494072584No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1444320.1024939069908527No Hit
AGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1432460.10165228066364576No Hit
TGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAAT1422610.10095329083877325No Hit
TGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAG1420170.10078014005982004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)