FastQCFastQC Report
Thu 29 Jun 2023
SRR1042868.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042868.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54567011
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT10921162.0014217014745412No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10903921.9982622834151573Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA9389101.7206549942785028No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG8445331.5476988468362323No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA8064821.4779662386125567No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA7909151.4494380130148599No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAA7471671.3692650308443686No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG7328241.342979918764471No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA5873461.0763756145631653No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA5541751.015586138665356No Hit
ATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG4875010.8933987606541248No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC4591370.8414186366191104No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA4218920.7731631113164692No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG4170580.7643042790084287No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA4167730.7637819854197255No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG4064970.7449500944810776No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG3213880.5889785680216203No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA3027900.5548957042928373No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA2860600.5242361543314147No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG2810530.5150602806519858No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG2687440.4925026954472548No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA2678970.4909504755538104No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA2677600.49069940810941615No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA2642110.4841954784732482No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2551930.46766900976122733No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT2466260.4519690477457158No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA2219520.4067512512275961No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2088320.38270742005641467No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2056820.3769347014444313No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC2047050.3751442423701749No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG1919790.35182245917776217No Hit
ATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGC1728090.3166913430534064No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG1714930.3142796294999556No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1678900.3076767389732965No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1608930.2948539732183608No Hit
TAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGA1547910.28367139259286167No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1501570.27517908210145503No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC1498670.27464762546733595No Hit
ATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAGAG1403210.25715353915940165No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA1373300.2516722053916422No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA1346260.24671683043075238No Hit
TTGCTCGAATATATTAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1301000.23842244172032806No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA1273850.23344690805952337No Hit
AATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCG1178770.2160224608967495No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1124290.20603840661164308No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG1121250.20548129345035962No Hit
AACGCGATGTACACGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA1112750.20392357572966568No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA1079350.19780266139188016No Hit
TCGACCCGCAACGCGATGTACCTGTAGGCACCATCAATAGATCGGAAGAG1076280.19724005040334716No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG1016520.18628837852232735No Hit
AGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCAATAGATCGGAAG1015920.1861784219773372No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT970050.17777224411283954No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG957590.17548881319521056No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT932720.17093111440536848No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA909330.16664464175983543No Hit
AGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAGAGCAC887060.16256342133161736No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG841920.15429102393019106No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAG834640.15295688451764383No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA787830.14437844139932823No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCA785760.1439990913191122No Hit
TTGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG766070.14039068403435181No Hit
TTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAAGA762460.13972911215532768No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT754960.13835465534295072No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC707820.12971573612489054No Hit
ACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACCATCAA706500.1294738317259122No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG704550.12911647295469417No Hit
TTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG698070.12792894226880047No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA672820.12330160433379794No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA660530.12104932777058286No Hit
TTTGCTCGAATATATTAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA656610.12033094500998047No Hit
TGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAGA653640.11978666011227919No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT653340.11973168183978411No Hit
CAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG650530.11921671868741354No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA647580.1186760990078786No Hit
ATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCAATAGATCGG644390.11809149671034758No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC615290.11275860427832486Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA612910.11232244331653056No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC603030.11051182554235929No Hit
ACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGA600700.11008482762598085No Hit
GATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAG597210.10944524705595474No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCAA590540.10822289679748082No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA590500.10821556636114812No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT588600.10786737063534597No Hit
TTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGC576320.10561692668121403No Hit
GTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAG569120.10429744814133213No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC563970.10335365446349992No Hit
TAACCGGGATTGCCTTAGTAACGCTGTAGGCACCATCAATAGATCGGAAG553560.10144590840792068No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT549970.1007880017470629No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)