FastQCFastQC Report
Wed 28 Jun 2023
SRR1042869.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042869.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53122756
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA31098315.85404680434878No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA15978493.0078428159864297No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA12992652.4457786038058718No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG12779572.405667733052103No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA12543092.3611519703533457No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT10147011.9101060946461437No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT10084401.8983201850446163No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC9276031.746149992669808No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA8778361.652466976675683No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT8087541.5224247778108502No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG7776021.4637832419688466No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA7708501.4510730580318536No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC5893621.1094341566164225No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA5382311.0131835027535092No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG4249440.7999283772099474No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT4118370.7752553350206454No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA4064970.7652031457102866No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT3108050.5850694192146206No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT3104940.5844839827210773No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT2490070.46873885835290624No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG2428010.4570564825364106No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT2408810.4534422122225737No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG2251170.4237675470000088No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA2237180.42113402399529115No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG2196970.4135647630932401No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA1868340.3517023853205206No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA1852010.3486283731213042No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG1838580.3461002663340735No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAA1745960.32866517693472075No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA1702740.32052930386367756No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA1700390.3200869322367236No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1694400.31895935519610463No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG1681600.3165498416535467No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC1656150.31175905105525775No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA1618590.30468863475381436No Hit
TTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1610040.30307915500468385No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG1468490.2764333236024125No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA1390340.2617221139656233No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC1390080.26167317072179014No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG1332360.250807770590818No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA1244780.23432142714884746No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGA1242260.2338470541701564No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA1217960.22927274330420658No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1197380.22539869731156267No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATC1192880.22455160270675717No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1156450.21769390127274268No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1110540.2090516538712713No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG1092210.20560115518103014No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1089260.20504583760676875No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA994340.18717778874273766No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCA967580.18214039949282754No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA921040.17337955884668332No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG918630.1729258926249986No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA878160.1653076884791143No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATC866350.1630845357496136No Hit
ATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG859400.16177624519330286No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG853000.1605714884220239No Hit
TTGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG802950.1511499139841314No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG802540.15107273425347134No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT800390.1506680112756198No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC794150.14949337342362282No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATC793040.14928442342110412No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC770880.14511295310055072No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCA751560.14147609359725238No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCAA740050.13930941384140538No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT725160.13650647191572665No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG715080.1346089800009623No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA699210.1316215596946815No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG680190.12804117316503685No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA659780.12419912852413004No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCA654690.12324097040447224No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA622950.11726612979191064No Hit
TCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGA617140.11617243653548398No Hit
TCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGA610870.11499215138612161No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC597690.112511105410269No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC595650.11212708918942384No Hit
ATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGATCGGAAGAG589190.11091103782341412No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA582480.10964792564602634No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA557920.10502467153624334No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA543790.102364794477154No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA540230.10169464852313008No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG533510.10042965391328718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)