FastQCFastQC Report
Wed 28 Jun 2023
SRR1042870.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042870.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52714529
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA34860026.612981404045174No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA16196223.072439478687176No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA12547722.3803153016884586No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA10148831.9252434181855251No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC8888451.6861480446880215No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG8269951.568817962880784No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT7437871.4109715368982998No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT6612411.2543809316782477No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG6136231.1640490992530732No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG5182250.9830781187478692No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA5060310.9599459761842889No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG4862280.922379482893606No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT4234840.8033534739540212No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA3794240.7197712038743626No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA3551750.6737706031671078No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA3425920.6499005236298326No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC3369700.6392355322002403No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA2976040.5645578280705116No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG2886980.5476630550943555No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2680170.5084309868347681No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2536690.4812126842677471No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT2141920.40632441200413644No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2085510.3956233773804561No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCA1966370.37302239767711853No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1834620.3480292880924726No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT1716590.32563887652301704No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAA1701960.3228635505782476No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA1687630.3201451349399328No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1577290.29921352422593017No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1557500.2954593410101416No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1554590.294907311037532No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1553050.29461517146439836No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG1547050.2934769653353063No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCA1482020.2811407079061638No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG1476930.2801751297066507No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA1421830.26972260342115545No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG1414610.2683529620458147No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA1392490.2641567754498954No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG1379340.26166220701696874No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1297820.2461977797430382No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC1288830.24449236755961531No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1259060.23884496814910364No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATC1222010.23181654530196028No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1139890.2162382974151206No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA1120650.21258844976116548No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1107350.21006542617501145No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATC1064020.20184568091275176No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG1063800.2018039466880184No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATC1052460.19965273710403444No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1023810.19421780283761997No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATCA1008530.19131917122886558No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA1001370.18996091191481576No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG978950.18570781501244182No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA965180.1830956319461756No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA947760.17979104015137837No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT937370.177820046537834No Hit
ATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG896140.16999867342075656No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA861920.16350710446450162No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCA847950.16085698119393232No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG844820.1602632169965893No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG841590.15965048269709475No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA795170.1508445612783527No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG771450.14634485304800882No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA745410.1414050384477494No Hit
AAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGA739800.14034081571704832No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA724640.13746494823087577No Hit
GCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG722410.13704191495289658No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA703090.1333768912172202No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA692790.1314229706956122No Hit
TTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAA678220.12865902681213373No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA670840.1272590332733505No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC643580.12208778342684234No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG613020.11629052020933356No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC599150.11365936704091581No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG592860.11246614761558432No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA591030.11211899474621123No Hit
TTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG553290.1049596781942223No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA545840.10354640558393303No Hit
GTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAA535600.10160386712361597No Hit
TTGGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA528700.1002949300751601No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)