FastQCFastQC Report
Tue 27 Jun 2023
SRR1042874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47268306
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG25710985.439369881374636No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT20708364.381024359112849No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC18034253.815294332739574No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA12444652.632768350107575No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA12120492.5641896284584433No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG11088322.3458255516920787No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG10725462.269059525848039No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA8798741.8614460183954975No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC7342031.5532670030527431No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG6180761.3075907564785587No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG4376710.9259290992996448No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA3737470.7906926048925891No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG3371730.7133172912945093No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA3018440.6385758778831634No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG3004090.6355400170253616No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2969740.6282729912089509No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG2859780.6050100462665194No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA2729450.577437659813745No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2530850.5354221917747591No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA2352510.4976928938388442No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA2275350.48136905942853125No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT2047350.4331337789003905No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1904840.4029846129878232No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1820110.3850592826406768No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1657780.3507170322541282No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATAGA1606040.3397710085062071No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC1436780.3039626594615005No Hit
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1373350.29054351979527254No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG1333280.28206638080069973No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGAT1325080.2803316031676701No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA1267160.26807814944753894No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGA1200470.25396932989305776No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1193450.252484190992586No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAG1162990.24604012676062476No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGA1158510.2450923458098964No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA1147660.2427969388198511No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1110680.23497351481138332No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT1085750.2296993676904774No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT1034220.21879777117462174No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT1027630.21740360232076014No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT1013430.21439947520014788No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCG988900.2092099513784141No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG969050.20501052015699484No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT915060.19358849035123027No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGGAA886940.18763947241942625No Hit
TTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATAGATCGGA874830.18507750203698858No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATAG863630.18270804966016765No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA860830.18211568656596241No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAG793480.16786723856784713No Hit
AAGAGGTGCACAATCGACCGATCCTGATCTGTAGGCACCATCAATAGATC790140.16716063401975947No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCCTGTAGGCACCATCAATAGAT782020.16544278104656426No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG765860.1620239997600083No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG758630.16049443362747123No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC705240.14919933877046493No Hit
TCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGA693930.14680661498637163No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGGAA691870.14637080499563493No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATAG681150.14410290057782058No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG674870.14277431478081742No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAAT674830.14276585245090018No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATAGATCGG669980.14173979494843753No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGA655830.1387462457402218No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGG654110.13838236555378142No Hit
TTTTCCACGTTCTAGCATTCCTGTAGGCACCATCAATAGATCGGAAGAGC649390.13738381062354973No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA638380.1350545543138356No Hit
AATAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG631150.1335249881812985No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC621010.1313797875472838No Hit
TATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGG620820.13133959148017701No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGAT598250.12656472182438694No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATA568020.12016931598944967No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAATA562180.11893381582153588No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA561840.11886188601723953Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG559800.11843030719146146No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATAGAT543780.11504114405961577No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA542250.11471745994028218No Hit
TCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG541800.11462225872871348No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATAGATCGGAA530430.11221684144974435No Hit
TGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAA528720.1118550768457833No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATA519730.10995316819688862No Hit
CCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATAGATCGGAAG517760.10953639844846566No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATA513050.10853995910071326No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGG510780.10805972187791117No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATAGAT509390.10776565591328786No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT498490.10545967101084604No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATA477070.10092809334017597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)