FastQCFastQC Report
Tue 27 Jun 2023
SRR1042875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52378814
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA30090745.744830343046713No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT10555672.0152556336995335No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG9636101.8396941939158837No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA9327721.7808192449718314No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC8194131.5643977734967422No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT8019771.5311095054576835No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG7879841.5043945057633417No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG7389041.4106924986884963No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA6900531.3174276912799134No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA6194341.182604096381411No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA5207000.9941042193127931No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT5142390.9817690793838898No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC4909230.9372548985167934No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT3839940.7331093827363101No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA3170170.6052389807833373No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC3034480.5793334686806769No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT2921410.557746496512884No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG2879670.5497776257400558No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA2704380.5163118049981047No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG2687120.513016579565929No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAA2467970.47117714425530904No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT2424290.4628378947259096No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT2244600.42853203969070397No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA2118540.4044650571889619No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA1729600.3302098440029589No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC1701200.32478780447377065No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1659540.316834207051729No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA1541480.29429455962863155No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1527310.29158926737058233No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1498220.28603549519086097No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC1490440.2845501618268791No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA1460490.27883220112620344No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATC1435030.27397145723841704No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG1400520.26738291554291393No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1356320.2589443892334026No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATC1345020.25678702843481716No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1301240.24842868721693467No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1258890.24034335714435995No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA1255010.2396025996312173No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1226250.23411183002349004No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG1224090.233699449552256No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG1222390.23337489084804403No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA1218370.2326074049710251No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG1204620.22998229780460475No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1191820.22753856167877343No Hit
AATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAA1154740.22045936358925577No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATC1100280.21006203004138277No Hit
TTGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1050130.20048754826712956No Hit
ATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCG1029330.19651647706265363No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCA997420.19042431926771003No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA989770.18896380509875615No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA987780.1885838804979433No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA962030.18366777071355606No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCA934980.17850346897888905No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA916830.17503832751921417No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA892150.17032649880159562No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG885960.1691447232844944No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG854300.16310029471075843No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCAT850220.16232135382064972No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT848560.16200443179183094No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA829600.1583846476554433No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA742410.14173860446706563No Hit
TTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG731040.13956787948654203No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC725700.13854838332154676No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA724350.13829064552702547No Hit
AAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGA719690.13740097284371502No Hit
AAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGA683840.13055660252253898No Hit
AAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA651320.12434798542784874No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG633820.12100693994331373No Hit
AGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACCATCAATAGATC609530.1163695688107791No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC607820.11604310093771883No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG606430.11577772646780433No Hit
TCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGA605300.11556199038794579No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACC598460.11425611889570467No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA578360.11041868951061015No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAG571630.10913381887570041No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC565420.10794822502090252No Hit
TATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCG531530.10147805179399443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)