FastQCFastQC Report
Mon 25 Sept 2023
SRR1042876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50131630
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGG6884181.3732208587672095No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAG5901371.1771749691761468No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAA5662241.1294745453120116No Hit
CCGGAGATGGGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4840350.9655281505907548No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAG4828080.9630805940281614No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG4459040.8894663907796335No Hit
AGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAG3940170.7859648688861702No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGAT3868290.7716266157713204No Hit
TATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGG3768120.751645218796995No Hit
ATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA3351580.6685559595808075No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG3158640.6300692796144869No Hit
TAGAGTGTTCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGA3096700.6177138066326588No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAG3083460.6150727594534628No Hit
TTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA2987630.5959570833822878No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGA2975980.5936332012344303No Hit
TTCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAA2794010.5573347605094827No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2766800.5519070495014824No Hit
CCGGAGATGGGGTCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2689260.5364397686650124No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2483410.4953778682241132No Hit
GTTACGATCTGCTGAGATTCTGTAGGCACCATCAATAGATCGGAAGAGC2392350.4772136872469536No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAG2390590.4768626114889941No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGC2283760.45555271193057156No Hit
AGTAGAGTAGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2165520.43196680419128597No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAGAT2141050.4270856543064728No Hit
ATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGAGCA2105830.4200601496500313No Hit
CGTGGGTTAGTCGATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1987750.3965061578887421No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1950200.38901587680272914No Hit
TTCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGA1926340.3842564065840269No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGCTGTAGGCACCATCAATAGA1856310.37028718196475957No Hit
AAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1792110.35748089579373343No Hit
AAAGATTGGAAATCTTTTGGGCCTGTAGGCACCATCAATAGATCGGAAG1750440.34916877827431503No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG1731750.3454405930946191No Hit
ATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1724030.3439006471562963Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGA1715190.3421372893719993No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAA1701100.3393266885596978No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG1697070.3385228048639153No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1662970.3317207120534481No Hit
TAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAA1613120.3217768901589675No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAA1550000.30918603683941653No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGCTGTAGGCACCATCAATAGATC1480870.29539633959637857No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG1404970.28025619753437103No Hit
TCCGGAGATGGGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1398480.27896160567689504No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1374350.274148277245324No Hit
AAGCAGTAGAGTAGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1358620.2710105376585601No Hit
ATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGA1328050.2649125911126369No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1308290.2609709678300905No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1286650.25665433180608727No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG1240190.24738672969540387No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC1231860.24572510409097012No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAA1212400.24184332326716687No Hit
TAGCCTGTGGGAATACTGCCCTGTAGGCACCATCAATAGATCGGAAGAG1196840.23873949440702405No Hit
AGAGTGTTCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAG1166860.23275923802996232No Hit
TTAGCCTGTGGGAATACTGCCCTGTAGGCACCATCAATAGATCGGAAGA1149760.22934821788160487No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATA1135730.22654958556105198No Hit
TTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGC1122510.22391252787910548No Hit
TTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG1080250.21548272019082565No Hit
TTATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCG1078240.21508177571724676No Hit
AAATCCATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1065950.2126302296574039Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGC1017780.2030215255318848No Hit
TTTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAG1005410.20055402148304374No Hit
AAAATCCATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1005020.20047622628667766No Hit
TATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAA1000300.19953470493578604No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAG994520.19838174023066873No Hit
TAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAA968510.19319339905764085No Hit
AAATGTGAATTGCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACA930460.18560338054038938No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGC928920.18529618925217473No Hit
AAATGTGAATTGCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC911940.18190910608731453No Hit
ATGTCTAAGTATAAGCACTGTAGGCACCATCAATAGATCGGAAGAGCAC909580.18143834541186873No Hit
TCGTGGGTTAGTCGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA909290.18138049770175038No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCAC905640.18065241445370916No Hit
TGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG893750.17828065833885712No Hit
AAAAGATTAAGCCATGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC888700.17727331028334806No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA883520.17624003049571696No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC863290.17220465402780638Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC862120.17197126843870825No Hit
AAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC840110.16758082671558855No Hit
AAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC834160.16639395128385015No Hit
AGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT812150.16200350956073042No Hit
CGTGGGTTAGTCGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACA793610.15830524561040604No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTA776220.15483637775193027No Hit
TTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAG765000.15259826979493785No Hit
TATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCAATAGATCGGAA744960.1486007935508979No Hit
AATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC736790.14697108392446048No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGCTGTAGGCACCATCAATAGA722330.14408667741304243No Hit
TATCTGGTTGATCCTGCCAGTAGTCTGTAGGCACCATCAATAGATCGGA706250.14087912162441157No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA681900.13602190872309558No Hit
AGGATAAATCCATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA678140.1352718832401819No Hit
AATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG665950.13284028466658673No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA664000.13245130868475652No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA655460.13074779335920256No Hit
CTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA630990.12586664347438933No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAG630150.1256990845899086No Hit
GCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG629920.12565320537153887No Hit
TGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGA628050.1252801873787068No Hit
TGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCG615930.12286255204548506No Hit
TTCGGTGCCCGAGTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC613830.12244365483428327No Hit
TTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC612300.12213845829469339No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACCATCA606980.12107725202631552No Hit
AGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG606770.12103536230519535No Hit
AAAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT601410.11996617704231839No Hit
AAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAAT599830.11965100675960466No Hit
CCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG595910.11886906529869465No Hit
TCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGG594330.11855389501598092No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGAGCTGTAGGCACCATCAATA588360.11736303008699298No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC586790.11704985455290402No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG569090.11351914948706038No Hit
TTGGGCCAGCATCAGTTTTGGTGGCCTGTAGGCACCATCAATAGATCGG560280.11176177594863762No Hit
ATAGAGGGTGAGAATCCCGTGTGGCTGTAGGCACCATCAATAGATCGGA558640.11143463717417526No Hit
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATAGATCGGAAGAGC555490.11080629135737258No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATAGATCGGAAGAGCA555160.11074046465275515No Hit
TAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT553910.11049112107465885No Hit
AACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCAATAG553020.11031358844705429No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGG552420.11019390352956805No Hit
TAGCCTGTGGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG550610.10983285402848461No Hit
TGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATC545660.10884545345922325No Hit
GAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC540230.10776230495597292No Hit
TATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATA540020.10772041523485272No Hit
TAGGATAAATCCATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC537000.10711800115017205No Hit
TATGCATGTCTAAGTATAAGCACTGTAGGCACCATCAATAGATCGGAAG535450.10680881511333264No Hit
TATACAGTGAAACTGCGAATGGCTCATTCTGTAGGCACCATCAATAGAT533960.10651159756824184No Hit
TCCGGAGATGGGGTCTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC527880.10529879040438142No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC516330.10299485574277159No Hit
TTGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAG513070.10234456769109641No Hit
TAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAGA505970.10092829616750942No Hit
TTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAGA505770.10088840119501401No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)