FastQCFastQC Report
Thu 28 Sept 2023
SRR1042877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41529493
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG5395841.2992790448946727No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAG4861741.1706716477371877No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAG4167961.0036144674340233No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGG3956200.9526241989036562No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3749260.9027945513324711No Hit
TTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA3703260.8917180857469172No Hit
AGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAG3352480.8072528118751655No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG3284300.7908355635355336No Hit
ATTGCGCCCCTTGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3128760.7533826622925544No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAA2493940.6005226213573086No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGC2484160.5981676684567279No Hit
AAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAA2479860.5971322597172087No Hit
TTCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAA2385850.5744953351585582No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGAT2053530.4944750950848353No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAG2033250.4895918185179867No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGC1844640.4441759016899147No Hit
TAGAGTGTTCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGA1821840.4386858274431619No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGA1749850.4213511588017701No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1746660.420583029992685No Hit
TATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGG1738000.41849776494983937No Hit
GTTACGATCTGCTGAGATTCTGTAGGCACCATCAATAGATCGGAAGAGC1570360.3781312716723992No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGG1528900.36814800508159345No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1506430.3627373924357805No Hit
AAGATTCCGGAACCTGGATCTGTAGGCACCATCAATAGATCGGAAGAGC1405600.3384582614577067No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGA1389280.3345285241021363No Hit
TTCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGA1386280.33380614591177404No Hit
AAGATTCCGGAACCTGGATACTGTAGGCACCATCAATAGATCGGAAGAG1383750.3331969403045686No Hit
AGTAGAGTAGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1328600.31991722123841No Hit
TTTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAG1324960.3190407357007705No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG1232520.2967818557284097No Hit
AATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG1224210.2947808681411064No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGCTGTAGGCACCATCAATAGA1201290.2892618987667391No Hit
AAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCGGAAGAGC1195840.28794957838758106No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC1174380.28278216639919007No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG1162440.2799071012015485No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAG1154170.27791574532345004No Hit
TAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAA1129900.27207170576341977No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1114780.2684309196839942No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG1109370.26712823101404104No Hit
ACATTGCGCCCCTTGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1099350.26471548785823124No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAGAT1094720.26360061751777225No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1089130.26225458615639735No Hit
ATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1084260.26108192556070936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA1063780.25615049044783667No Hit
ATAGAGGGTGAGAATCCCGTGTGGCTGTAGGCACCATCAATAGATCGGA1058140.25479241944995573No Hit
ATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA1026460.2471641057597308No Hit
ATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGAGCA967440.23295251882800494No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA966640.23275988464390834No Hit
ATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGA963500.23200379547132924No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG961290.23147164353776242No Hit
ATCCGATTGGAAGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACA950460.22886385827055486No Hit
TTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGC934130.22493171298768322No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGG918700.22121628116192027No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC911510.21948498143235218No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATAGATCGGAAGAGCA894810.2154637428393359No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGC894570.21540595258410694No Hit
TCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGC881620.2122876867290434No Hit
AAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC848870.20440172481758925No Hit
ACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCG833620.20072963568324806No Hit
AAGCAGTAGAGTAGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC811020.19528771998251943No Hit
AGAGTGTTCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAG807160.19435826004425338No Hit
TAAGATTCCGGAACCTGGATACTGTAGGCACCATCAATAGATCGGAAGA788520.18986988355480283No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA787680.18966761766150142No Hit
TAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAA763670.18388618421130257No Hit
AAAGATTGGAAATCTTTTGGGCCTGTAGGCACCATCAATAGATCGGAAG758110.18254737663183127No Hit
TTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAG726090.1748371934133653No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGCTGTAGGCACCATCAATAGATC720340.17345263521517107No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA713640.17183932392336213No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAG681720.16415321997790824No Hit
TTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA658590.1585836841302156No Hit
ATTGCGCCCCTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG642050.15460097237401862No Hit
AAAGATTGGAAATCTTTTGGGCTGTAGGCACCATCAATAGATCGGAAGA623730.15018964955820674No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGC614610.14799361985950563No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTA612580.14750481061736054No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA604640.14559291634020188No Hit
TATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAA603690.14536416324658719No Hit
GTCAAGGATGCTGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA597280.14382068184651328No Hit
TGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGG589580.1419665778245836No Hit
TATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCAATAGATCGGAA574530.13834264723626655No Hit
TTATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCG570290.13732168606055462No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC564890.13602140531790263No Hit
TCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAG554410.1334978975062373No Hit
GCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG554210.13344973896021317No Hit
TGACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGAT552910.1331367084110562No Hit
TATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC548760.13213741858105515No Hit
AACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCAATAG540330.13010753586613735No Hit
TGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGA538440.12965243760620915No Hit
TGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATC526590.12679904375427845No Hit
TTAGCCTGTGGGAATACTGCCCTGTAGGCACCATCAATAGATCGGAAGA516920.12447057805401092No Hit
CCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG515920.12422978532389019No Hit
TAGCCTGTGGGAATACTGCCCTGTAGGCACCATCAATAGATCGGAAGAG514920.12398899259376944No Hit
TGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC514870.12397695295726342No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCA513640.12368077789921489No Hit
AGGATAAATCCATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA511250.12310528327422635No Hit
TGAACGCACATTGCGCCCCTTGGTCTGTAGGCACCATCAATAGATCGGA511210.1230956515650215No Hit
TAAGATTCCGGAACCTGGATCTGTAGGCACCATCAATAGATCGGAAGAG499850.12036024615084996No Hit
CTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA495780.11938021973925854No Hit
AGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA494890.1191659142094511No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG494650.11910812395422213No Hit
TGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCAATAGATCGGAAGAG492810.11866506533079997No Hit
AAAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT487920.11748758888050956No Hit
AAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC487400.11736237666084678No Hit
TAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC483210.1163534551216409No Hit
AAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATC482360.11614878130103828No Hit
AAAGATTCCGGAACCTGGATACTGTAGGCACCATCAATAGATCGGAAGA481160.11585983002489339No Hit
TGTTCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA477980.11509410914310947No Hit
AAATCCATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT474600.11428022971530136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCA468980.11292697457202282No Hit
TGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGC468200.11273915624252866No Hit
TATACAGTGAAACTGCGAATGGCTCATTCTGTAGGCACCATCAATAGAT457960.11027343868609232No Hit
ACCTGCGGAAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG451600.10874199692252444No Hit
ATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATCGG450360.10844341393717472No Hit
AAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC448350.10795942054963203No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA444030.10691919595551046No Hit
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG442340.10651225624160642No Hit
TATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAG440970.106182370201341No Hit
ATCCGATTGGAAGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC439040.10571764023220799No Hit
TCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGG438570.10560446764905124No Hit
TCAAAGCAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT435720.10491820836820716No Hit
ATGTCTAAGTATAAGCACTGTAGGCACCATCAATAGATCGGAAGAGCAC435660.10490376080439989No Hit
AGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC434570.1046412967285683No Hit
GTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA429800.10349271540589237No Hit
TAGTCAAGGATGCTGGCACTGTAGGCACCATCAATAGATCGGAAGAGCA422730.10179031080393879No Hit
CCGGAGATGGGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC422420.10171566505760136No Hit
TGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG419630.1010438533405645No Hit
TGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC419550.10102458992215485No Hit
TTTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGG417640.10056467580762424No Hit
GAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC417610.10055745202572063No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC415710.1000999458384912No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)