FastQCFastQC Report
Wed 28 Jun 2023
SRR1042878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32596618
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA457633214.039284688982152No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC13401444.111297681250245No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC9966073.0573938682841266No Hit
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC6991782.144940312519538No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGC6519812.000149217934204No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGG6254451.9187419995534507No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG4042881.2402759083779797No Hit
TTGTGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA4036271.2382480906454774No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCT2880490.883677564341184No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG2673340.8201280267787291No Hit
ATGTACACGGAGTCGAGCACCTGTAGGCACCATCAATAGATCGGAAGAGC2452930.7525105825395751No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCCTGTAGGC2432410.7462154509403399No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA2125190.6519664095213804No Hit
TCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGC1955470.5998996583019748No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT1946180.5970496693859467No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1945820.596939228480697Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGCA1921710.589542755631888No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA1858010.570000851008531No Hit
ATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAG1736030.5325797909464105No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA1571640.4821481786852857No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT1460120.4479360404812548No Hit
TAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCG1408910.43222582170947915No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC1291110.39608710326942503No Hit
TGGTCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC1270470.3897551580351066No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATCTGTAGGCACCA1200400.36825906294941396No Hit
TTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAG1131290.34705747694438727No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAG1031830.31654510906622274No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA1022110.3135632046244798No Hit
GCCTTGGGTGCTTGCTGGCGAATTGCAATGTCCTGTAGGCACCATCAATA1001420.3072159203755433No Hit
CTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA990960.30400699851745355No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCCTGTAGGCAC939910.2883458645924556No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCT892840.273905716231052No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTT892240.2737216480556357No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAGA887460.272255238258153No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGCTGTAGGCAC865470.265509139629148No Hit
TTTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC836540.2566339857711619No Hit
TCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATA833850.25580874678471244No Hit
ATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACCATCAATAG786640.2413256491823784No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC776740.23828852428801048No Hit
ACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCA770910.23649999518354942No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCCTGTAGGCA764600.23456421153875534No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC743630.22813102880795794No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA741660.22752667163200793No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG716110.21968843516219994No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCCTG683300.20962297376985553No Hit
GTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA661410.20290755317008657No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT639990.19633631930772696No Hit
TTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCC637270.19550187691250667No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG628770.19289424442744335No Hit
TAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAGAT591230.18137771225223426No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGT587320.18017820130910514No Hit
AGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCCTGTAGGCA586850.18003401457169574No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACC578070.1773404836047715No Hit
TTGTGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGC565940.17361923865843995No Hit
TTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGC546280.16758793811063466No Hit
ATGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA536540.16459989806304445No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGTCTGTAGGC534510.16397713406955286No Hit
TCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTC509210.15621559267283497No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT504290.15470623363442182No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACC486690.14930690048887893No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC484080.14850620392581831No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGGCTGTAGGC481570.14773618539199374No Hit
AGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCA480120.14729135396807116No Hit
TAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAA471940.14478189117656315No Hit
AACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCA471110.14452726353390402No Hit
ACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCAC470140.14422968665031446No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAAT460860.14138276553721002No Hit
TCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCG459970.14110973107700928No Hit
AACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCG450020.13805726716802338No Hit
TAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG450000.13805113156217616No Hit
CCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACCATC429900.1318848476857323No Hit
TCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACCATCAATAGA425460.13052274318765217No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACC421210.1292189269451205No Hit
TTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGT414960.12730155011786806No Hit
TGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGG413330.12680149824132061No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA404800.12418466234748649No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTCTGTAG403670.12383800061711923No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCT394740.12109845260634094No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC392140.12030082384620391No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCCTGTAGGCA390850.11990507726905902No Hit
TTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTA386180.11847241330373598No Hit
AGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTCTGTAGGC379300.1163617648922965No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAGATC368020.11290128319447128No Hit
TTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCG365450.11211285784310508No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC364930.11195333209107768No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA362210.1111188896958574No Hit
AACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCTGTAGGCACC357290.10960953065744428No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGC356220.10928127574461866No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA355100.10893768181717502No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT332090.10187866728996241No Hit
ATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGCACCATCAATA331250.10162097184437968No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTGT326760.10024352833168151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)