FastQCFastQC Report
Wed 28 Jun 2023
SRR1042879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37151439
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGG13606213.6623641953680446No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG8873002.3883327910932333No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG6214221.6726727597280957No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA4207871.1326263835971468No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT3849571.0361832821603492No Hit
TAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCG3397440.9144840930656819No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA3340900.899265301675125No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT3099400.8342610901289719No Hit
GCCTTGGGTGCTTGCTGGCGAATTGCAATGTCCTGTAGGCACCATCAATA2842120.7650093984246478No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA2725900.7337266263091451No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA2531950.6815213806388496No Hit
TTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAG2238790.6026119203619542No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2204780.5934574970299266Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCCTGTAGGCAC1999140.5381056706847883No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAG1972650.5309753950580488No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC1953100.5257131493614554No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCT1770570.47658180884998824No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA1744650.46960495931261237No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG1520690.40932196462161263No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG1468700.39532789026018617No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCCTG1430020.38491644966968847No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGT1309940.3525946868437586No Hit
TCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTC1256790.3382883769320483No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT1249460.3363153712565481No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAAT1180270.3176915973564308No Hit
TAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAA1036800.2790739814950371No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACC990260.26654687588278886No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC981320.2641405087969809No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCCTGTAGGCA962000.25894017187328866No Hit
AACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCG930730.2505232704445176No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA913580.24590702933471836No Hit
TAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG904580.24348451213424063No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTCTGTAG885550.2383622340981193No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCT880910.23711329189698413No Hit
AACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCTGTAGGCACC852730.22952812137371045No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGC843580.22706522888655806No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACCTGTAGGCA823900.22176799127484673No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC799330.21515451931754243No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA784460.21115198256519752No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC766600.20634463176513834No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA761290.20491534661685648No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC750880.20211330172163722No Hit
TTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATACTGTAGGCAC744410.20037178102307154No Hit
CCTTGGGTGCTTGCTGGCGAATTGCAATGTCCTGTAGGCACCATCAATAG727100.19571247294081934No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT721150.1941109199027257No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA720950.19405708618715953No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTGT697470.1877370079796909No Hit
TAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT667720.17972924278922278No Hit
ATGTACACGGAGTCGAGCACCTGTAGGCACCATCAATAGATCGGAAGAGC656640.17674685494685685No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCCTGTAGGCACC625210.16828688654563287No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCTGTAGGCACCATC617640.16624928041145323No Hit
ATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGC608210.1637110207225082No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC607340.16347684405979537No Hit
TATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCACTGTAGGCAC599460.1613557956664882No Hit
TATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGG594360.1599830359195508No Hit
TTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTG591740.15927781424563392No Hit
TAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCT591210.15913515489938357No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCCTGTAGGCACCATCAATAGA587470.15812846441829617No Hit
TCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGG583270.15699795639140654No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC567800.1528339184923631No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAG564860.15204256287354037No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG564540.1519564289286345No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGACTG561100.15103048902089636No Hit
TAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCC541420.14573325140918497No Hit
TCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGTA538900.1450549465930512No Hit
ATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGA533500.14360143627276456No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCTG533500.14360143627276456No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCA531180.14297696517219696No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTG529450.14251130353254957No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA524310.14112777704249896No Hit
AACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCCTGTAGGC520020.13997304384360457No Hit
TAGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAG512690.13800003816810433No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC510660.13745362595510768No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACCTGTAGG509760.1372113742350599No Hit
ATGGATGGCGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGACTG508110.13676724608163898No Hit
ACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCCTGTA507060.1364846190749166No Hit
ATCACCCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAG502110.13515223461465384No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT495370.13333803840007383No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG488610.1315184588139372No Hit
ACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCACC470250.1265765237249626No Hit
AACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCG460050.1238310042310878No Hit
GCCTTGGGTGCTTGCTGGCGAATTGCAATGTCTGTAGGCACCATCAATAG458900.12352146036658229No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA458840.12350531025191246No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGAT458150.12331958393320916No Hit
TAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCTGTAGGCACCAT455440.12259013708728751No Hit
TAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACC445730.11997651019654985No Hit
TAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCCTGTAGGCAC443810.1194597065271146No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGAC439900.11840725738779592No Hit
ACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCAC423440.11397674259669996No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCCTGTAGGCAC422910.1138340832504496No Hit
AAATGGATGGCGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGAC417420.11235634775815817No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA415680.11188799443273248No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGC415600.11186646094650599No Hit
ACCCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCC413890.11140618267841523No Hit
ATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA409790.11030259150930868No Hit
TGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGTAG406390.10938741834468377No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCCTGTAGGCACC402620.10837265280626142No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG398050.10714255240557438No Hit
TTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCCTGTAGGC396480.10671995773837994No Hit
AAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAAA393660.10596090234889691No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTCTGTA392020.10551946588125428No Hit
AGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCA373090.10042410470291609No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)