FastQCFastQC Report
Wed 28 Jun 2023
SRR1042880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34905832
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA28787748.247257936725301No Hit
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC17083104.8940532344279895No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC9756162.7949942576930984No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT9678192.77265701616853No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC8240512.360783149360256No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGG6593231.8888620102222458No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG6328051.8128918972623258No Hit
TCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGC6325511.8121642251644365No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA4582351.3127748967565076No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG4215261.2076090895068767No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT3242210.9288447844474814No Hit
TTTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC2967600.8501731172028789No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGC2781920.7969785679367276No Hit
TTGTGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA2438270.6985279709132847No Hit
TTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAG2314200.6629837672971096No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCCTGTAGGC2182720.6253167092536285No Hit
GCCTTGGGTGCTTGCTGGCGAATTGCAATGTCCTGTAGGCACCATCAATA2030710.5817681125606747No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAG2001850.5735001532122197No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCCTG1984950.5686585553955568No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC1864640.5341915356723197No Hit
ATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAG1736700.49753863480463667No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG1584630.45397284900700835No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCT1232160.3529954535964076No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC1212240.34728867084445947No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAGA1193530.3419285350367813No Hit
TAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG1190430.34104043129526324No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCT1163780.33340560396898716No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT1160700.33252322992902733No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1129570.3236049494537188Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCG1120920.3211268535298056No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG1105030.3165746056418309No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGC1062110.3042786660979747No Hit
TTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCC1017090.2913811078905095No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATCTGTAGGCACCA999920.2864621591028112No Hit
TCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCG954940.27357606029846243No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA944610.27061666944366203No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA942330.2699634834660294No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGC938740.26893500203633597No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGCA903690.25889370005562395No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG881550.2525509204307177No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG816830.2340096061884444No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA810790.23227923631787378No Hit
TCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATA810050.23206723736022106No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCCTGTAGGCA787450.22559267459947666No Hit
TATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG784640.22478765153055225No Hit
TGGTCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC775370.22213193485833543No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC718780.20591974429946264No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGT688290.19718481427401588No Hit
TTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTG683450.19579822649693612No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCCTGTAGGCAC677530.19410223483571457No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA667760.1913032756245432No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGCTGTAGGCAC662410.18977058045772982No Hit
ACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCA640510.18349655725152175No Hit
CCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGC629390.18031084318517318No Hit
AACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCG623130.17851744659746258No Hit
TAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAA594760.170389864937183No Hit
TCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTC586690.168077930358457No Hit
CTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA573460.16428773277772035No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC560960.1607066693038573No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACC547820.15694225538013246No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA540170.1547506445341283No Hit
TTTTCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG509560.14598133629933246No Hit
ATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA508830.14577220219245884No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGTCTGTAGGC507120.1452823127092344No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGCA503560.14426242583187818No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTGT499720.14316232313270746No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAAT498120.14270394700805297No Hit
TATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGG495650.14199632886561764No Hit
TCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGTA483170.13842099509331277No Hit
ATGTATTTATTAGATTGATGATTCATAATAACTTTTCGAATCGCATCTGT471010.1349373365459388No Hit
GCCTTGGGTGCTTGCTGGCGAATTGCAATGTCTGTAGGCACCATCAATAG465720.13342183048379996No Hit
TTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGC464770.13314966965978636No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC447260.128133315945599No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGCA447200.12811612684092447No Hit
CTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCT446680.12796715460041175No Hit
TAGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAG446620.1279499654957372No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCT442490.12676678212397285No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG438010.12548332897494033No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT435760.124838737549645No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC416290.11926087308275592No Hit
ACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCAC413240.11838709359513332No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTG395510.11330771316380597No Hit
ATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACCATCAATAG391990.11229928568956614No Hit
ATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGA386790.11080956328443911No Hit
TTATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGC386730.11079237417976456No Hit
AAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCA385650.1104829702956228No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGACTG382710.10964070416657021No Hit
ATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGC382640.10962065021111658No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACC381690.109348489387103No Hit
TAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCATCAATAGAT377520.10815384661222228No Hit
ATGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA375590.10760093041185781No Hit
TTATCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG371810.10651801681736163No Hit
TAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCC369770.10593358725842718No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTT369240.1057817501671354No Hit
TGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGG368680.10562131852350633No Hit
ACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCCTGTA368510.10557261606026179No Hit
TAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT362520.10385657044358662No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG362510.10385370559280753No Hit
TTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGACTGTA360500.10327787058621034No Hit
GTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA359740.10306014192699947No Hit
ACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCAC355550.10185976945056058No Hit
AACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCG349780.1002067505510254No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTCTGTAGGCACCA349620.10016091293855996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)