FastQCFastQC Report
Wed 28 Jun 2023
SRR1042881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54568894
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT21767503.988994169462185No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGG15672322.8720244907290957No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG15313962.8063533778053116No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG10570701.937129237033831No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA10533451.9303030037588813No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT7927261.452706738018183No Hit
GCCTTGGGTGCTTGCTGGCGAATTGCAATGTCCTGTAGGCACCATCAATA5538171.0148950425859833No Hit
TTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAG5108150.9360919061324571No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC4830460.8852039405453224No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCCTG4768650.87387697467352No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAG4440560.8137529780244401No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG3524610.6459009412945037No Hit
TAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCG2951700.5409125572528555No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT2893140.530181168780881No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG2796610.5124916037330718No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCT2780010.5094495776293358No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGC2745310.5030906435450203No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC2700230.4948295268729471No Hit
TAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG2558810.46891366352413155No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2464540.4516382538374335Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA2443470.4477770797407035No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG2286400.4189932821434863No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGC2221010.4070102648589506No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG2199870.40313626294130134No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA2096950.3842757010981384No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA1920580.35195509001886677No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA1890990.34653258686166516No Hit
TATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG1806990.33113920175842304No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA1702120.31192129347536346No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGT1689050.30952615605513284No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC1610750.2951773220838964No Hit
TTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTG1590400.2914480912880514No Hit
TCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTC1582730.29004252862445773No Hit
ATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCCTGTAGGCAC1582420.2899857197032434No Hit
TAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAA1480050.2712259478815898No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCTGTAGGCACC1465700.2685962445931193No Hit
AACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCG1452380.26615529352674805No Hit
GCCTTGGGTGCTTGCTGGCGAATTGCAATGTCTGTAGGCACCATCAATAG1274310.23352314965372029No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGCA1242390.2276736633144883No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA1221540.2238528052263621No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACCATCAAT1210740.22187365571308812No Hit
ATGTATTTATTAGATTGATGATTCATAATAACTTTTCGAATCGCATCTGT1210360.22180401897095442No Hit
TAGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAG1198410.21961412668543365No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTGT1191770.21839731624393927No Hit
TCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGCTGTA1153990.21147395803917157No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGCA1130560.20718030312287436No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCT1129630.20700987635923132No Hit
ATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA1119120.20508387067548042No Hit
TATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGG1115940.20450112109657198No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG989290.1812919279617432No Hit
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC981330.17983322146862643No Hit
TAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCC972590.17823157639955098No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGACTG969830.17772579374615874No Hit
ACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCCTGTA950330.17415232934719183No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAG948940.17389760547465008No Hit
CCTTGGGTGCTTGCTGGCGAATTGCAATGTCCTGTAGGCACCATCAATAG943660.1729300212681606No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAACTG928910.17022701614586508No Hit
ATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGC927390.16994846917733022No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA877080.16072893102799554No Hit
ACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCAC875700.16047603970129942No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT871260.15966238934584234No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC858780.1573753721305035No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA842510.15439382003967314No Hit
TAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT841920.15428569983478133No Hit
TTATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGC835860.15317517705233316No Hit
AAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCA834440.15291495554225454No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTCTGTAGGCACCA832790.15261258547772655No Hit
ATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGA821300.1505069903011045No Hit
TAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCCT799280.14647172434904032No Hit
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGC797470.14614003355098235No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGCT797180.14608688972145928No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA791150.14498186457654796No Hit
ACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAG787180.14425434387583522No Hit
AACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCG775040.14202963321924758No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGG772160.14150186001570783No Hit
TAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACC762120.1396619839867013No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG755790.13850198246642126No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCGG749130.13728150693323563No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC747960.1370670990692976No Hit
ATCACCCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAG737020.1350622939141849No Hit
TAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTA727340.13328838953562078No Hit
ATGTATTTATTAGATTGATGATTCATAATAACTTTTCGAATCGCATGGCC699590.128203074814014No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGCT686800.12585924867746082No Hit
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG683820.12531315001546486No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCT674070.12352641781598139No Hit
TAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGC673910.1234970970824514No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTTC669780.12274025564820866No Hit
TCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGACTGT666130.12207137641455588No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG660730.12108180165791887No Hit
AAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTA656900.12037993659904486No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCCTGTAGGCA643970.11801045482065296No Hit
TGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCACC643940.11800495718311609No Hit
ATGTATTTATTAGTTGATGATTCATAATAACTTTTCGAATCGCATGGCCC638920.1170850191686128No Hit
ACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTA634610.11629519190914882No Hit
ACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGA631320.11569228432593852No Hit
ACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCA626120.114739360486214No Hit
ACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATG622930.11415477836145992No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC612510.11224526559031965No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC604990.11086719111441035No Hit
TAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGT602480.1104072221071587No Hit
TCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATG592180.10851969988616593No Hit
CTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG588460.10783799283159376No Hit
ATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCT585690.1073303776323559No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCC583120.10685941335003052No Hit
ATGTATTTATTAGTTGATGATTCATAATAACTTTTCGAATCGCATCTGTA577340.10580020185125981No Hit
TGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG573720.1051368202551439No Hit
TCGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATG570140.10448076884241049No Hit
TAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACC564480.10344354789378726No Hit
TTTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAAC558740.10239166657839904No Hit
TCACCCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGG555470.10179242408687997No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG548180.10045649816542003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)