FastQCFastQC Report
Tue 27 Jun 2023
SRR1042900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21277427
Total Bases893.6 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCA7344513.4517848422179997No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAA7240013.402671761016969No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA4893612.2999068449394753No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCAT4415832.075359017798534No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAAT3353941.5762902159175542No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAAT3254391.5295035438260463No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATC3143391.4773355819761478No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCAT1767740.830805341266122No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATC1625870.7641290462422924No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCA1458830.6856233133827694No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAA1387030.6518786317537361No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCA1315080.6180634528789595No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATC1311990.6166112096166515No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1307660.6145761891228672Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCA1262060.593145026416963No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCA1136170.5339790379729654No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCA1124820.5286447463784038No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATA1101010.5174544835707814No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATC1074130.5048213771336167No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCA1057810.49715127679676685No Hit
GGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAAT1000030.46999573773652237No Hit
GGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCA988530.46459094889621755No Hit
CGCTGAGAAGACGGTCGAACTTGCTGTAGGCACCATCAATAG950140.44654835380236524No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAA934970.43941873234954587No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAA896660.4214137357867565No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATC894860.4205677688378393No Hit
GGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAA882780.4148903906473278No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAA843200.3962885174039136No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCAC818450.38465647185630103No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA787980.3703361313376847No Hit
GGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATA733280.3446281357233654No Hit
GCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATA706580.33207962598109253No Hit
GGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAG701970.32991301062858774No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA640300.3009292429954054No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCAT546620.2569013631206442No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCAT526250.2473278371487304No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCA508730.23909375884593564No Hit
CCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAAT504120.23692714349343086No Hit
CCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAA498060.23407905476540936No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCA486380.22858966923021284No Hit
AGAATTAAACTCCGAATGCCATAACTGTAGGCACCATCAATA483140.22706692872216175No Hit
CGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAG480710.22592487334112343No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACC465860.21894564601255595No Hit
CCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATCA461690.2169858225808976No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAA456240.21442442265223138No Hit
CGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCA448110.21060347193295503No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACC420530.1976413783489893No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG405630.1906386519385074No Hit
TGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCA405140.1904083609357466No Hit
CGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATC403660.1897127881110813No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATC403300.18954359472129784No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAA397990.18704799222199187No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCA396750.18646521499051555No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCAT394940.1856145482252154No Hit
GCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAGA391640.18406360881886707No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAA386900.18183589585338492No Hit
TGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAA379840.17851782548707604No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCTGTAGGCACCATCA379040.17814184017644613No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAAT373030.17531725053033903No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCA355870.1672523656173277No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAG349400.16421158441760839No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCA346370.16278754005359766No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCA342420.1609311125823625No Hit
GGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAA340320.15994415114195903No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTCTGTAGGCACCATC339900.15974675885387835No Hit
CCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCA339390.15950706821835178No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATTAA334680.15729345470201825No Hit
GGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAA334410.15716655965968065No Hit
GCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAG328570.1544218668920824No Hit
TGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAA324320.15242444492936105No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCA317820.1493695642804931No Hit
CTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAAT315170.14812411293903158No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGCTGTAGGCACCAT312230.1467423669224667No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATC310700.14602329501588704No Hit
CCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAA310190.14578360438036048No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCA310110.14574600584929748No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACC306610.14410107011529166No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCAT306280.14394597617465682No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATC302700.14226344190958803No Hit
CTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATCAA297030.13959864602049862No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATTA292990.13769992020181762No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAA289730.1361677800610008No Hit
TCGCTGAGAAGACGGTCGAACTTGCTGTAGGCACCATCAATA287990.13535001201038077No Hit
CCCCCCCGAGTGTTACAGCCCCCCTGTAGGCACCATCAATAG287950.13533121274484927No Hit
GGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAAT285900.13436775038636017No Hit
TCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATA281990.13253012218065652No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCA281360.13223403374853548No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACC280860.1319990429293918No Hit
GCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAAT279250.1312423724917491No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAA279060.13115307598047451No Hit
CCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCA278240.1307676910370789No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC277240.13029770939879148No Hit
GGGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATC273890.1287232709105288No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCAT272580.12810759496437235No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAAT270620.1271864309533291No Hit
TGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAAT270340.12705483609460863No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCAT264690.12439943983828496No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC261390.12284850043193664No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACC259280.12185683917515028No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTAGGCACCAT259270.12185213935876739No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGCTGTAGGCACCATC245740.11549328779273922No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATC243840.11460032267999322No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATC242090.11377785481299031No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCAC239020.11233501118344806No Hit
TGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATA238370.11202952311856129No Hit
GGCGCGGGACATGTGGCGTACGGAAGACTGTAGGCACCATCA237600.11166763725708No Hit
CTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATA237080.11142324680517056No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCAT236500.1111506574549639No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCAC236290.11105196131092354No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGTACCATCA234960.11042688573200134No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCA231420.10876315073246404No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCATCATCAA230830.10848586156587449No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG228800.10753179884015111No Hit
GCGCGGGACATGTGGCGTACGGAAGACTGTAGGCACCATCAA226640.10651663850145038No Hit
CGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAATA223880.10521948917977723No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACTATCAA223650.10511139340297114No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCAC222700.10466491084659814No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAAT221840.10426072663767098No Hit
CCCCCCCGAGTGTTACAGCCCCCTGTAGGCACCATCAATAGA221470.10408683343150467No Hit
CCCCCCCGAGTGTTACAGCCCCCCCTGTAGGCACCATCAATA219580.10319856813514151No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACC219040.10294477805046634No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCA218090.10249829549409334No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAAT217590.10226330467494966No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC214600.10085805957647041No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCTGTAGGCACCATC214460.10079226214711016No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)