FastQCFastQC Report
Tue 27 Jun 2023
SRR1042901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22678915
Total Bases952.5 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCA8070323.558512389150892No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA6436612.838147239407176No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAA5677302.5033384533607537No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCAT3936721.7358502379853709No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATC2959611.305005111576105No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCAT2523891.1128795182661957No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAAT2239980.9876927533790748No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCA2221540.9795618529369681No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATC2211170.9749893237837877No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAAT2166660.9553631644194619No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATC1869210.8242060962792973No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAA1478270.6518257156482133No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA1340810.5912143504219669No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATC1276380.5628047020767969No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCA1189480.5244871723360663No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCA1155020.509292441900329No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCA1109780.48934439764865295No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC963430.4248130918079635Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAA938760.4139351463683338No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCAT900950.39726327295640024No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATA898200.3960506929013138No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCAT872740.3848244062822229No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCAT868900.3831312035871205No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAG829490.3657538290522276No Hit
CGCTGAGAAGACGGTCGAACTTGCTGTAGGCACCATCAATAG812550.3582843359128953No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCA764130.3369341081793375No Hit
GGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCA754270.33258645750910043No Hit
GGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAA748540.33005988161250216No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAA737390.3251434206618791No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACC709500.31284565421229366No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCA704280.3105439567986387No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCAC692970.3055569457357197No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATC645860.2847843470465849No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAA635510.2802206366574415No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAA597520.26346939436917505No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATC594650.2622039017298667No Hit
TGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAA589810.2600697608329146No Hit
GGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAAT585680.25824868605927576No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCA575120.2535923786477439No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCAC570150.2514009157845514No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCA564940.24910362775291497No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCTGTAGGCACCATCA544690.2401746291654605No Hit
TCGCTGAGAAGACGGTCGAACTTGCTGTAGGCACCATCAATA537230.23688523017966248No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCA531400.2343145604628793No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCA530300.2338295284408447No Hit
TGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCA508430.22418620996639388No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCA493210.21747513053424292No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCAT492930.21735166783772505No Hit
CCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCA492250.21705182986046734No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCAC491040.21651829463622932No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCAT480700.21195899362910442No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCAC471590.20794204661025453No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACC470520.20747024273427545No Hit
TGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAAT459320.20253173487355985No Hit
TGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAA456880.20145584566104682No Hit
GGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATA453590.20000515897696164No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATC447230.19720079201319815No Hit
AGAATTAAACTCCGAATGCCATAACTGTAGGCACCATCAATA423560.18676378477541805No Hit
GCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATA422370.18623906831521703No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACC421210.18572758000107148No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCA410010.18078907214035592No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACC404790.178487374726701No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC404740.1784653278166085No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCAT392830.17321375383257973No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCAT389680.17182479849675347No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA388320.171225122542238No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC387750.17097378776718375No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATTA385520.16999049557705911No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCA369430.162895799909299No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAA369370.16286934361718802No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATC364740.16082779974262437No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGTACCATCA357930.15782501058802856No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCA340680.1502188266061229No Hit
TGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATA338890.1494295472248121No Hit
CGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCA335880.14810232323724482No Hit
TCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATC333370.14699556835060232No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACC331950.14636943610397588No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACC330380.145677163127072No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCA329510.14529354689146284No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGCTGTAGGCACCAT327630.1444645830719856No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTAGGCACCAT323670.14271846779266115No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATC313340.13816357616755476No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCA312550.1378152349880936No Hit
CCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA311350.13728610914587405No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATC310660.1369818617865978No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAA308130.1358662881359183No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATC302280.13328679965509815No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCA301140.1327841301049896No Hit
CGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAG300360.13244019830754689No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATTAA295440.13027078235444683No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCAT284890.12561888432493354No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACC284450.1254248715161197No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCAC284250.12533668387574978No Hit
CCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAA283770.12512503353886198No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGCTGTAGGCACCATC282620.12461795460673494No Hit
CCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAA279830.12338773702357454No Hit
CCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATCA278830.12294679882172493No Hit
CCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAAT278580.12283656427126255No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAA274920.121222730452493No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACTATCA272860.12031439775668282No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCAT269900.11900922067920798No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACC264070.1164385509624248No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCA258280.1138855187737156No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCA257220.113418124279755No Hit
TCGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCAT256080.11291545472964645No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATC254550.11224081928081656No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCGCTGTAGGCACCATC253880.11194539068557732No Hit
GGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCA252360.11127516461876594No Hit
AGGGGAGATACCATGATCACGAAGGTGGTCTGTAGGCACCAT250640.11051675091158461No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCA246580.10872654181207522No Hit
TCCTCGCCCGTCACGCACCGCACGTTCGTGCTGTAGGCACCA245150.10809600018343028No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCATCATCA239590.10564438378114649No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGTACCA239490.10560028996096155No Hit
GGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATC237590.10476250737744729No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCA235700.10392913417595154No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCA232640.10257986327829174No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCA232470.10250490378397734No Hit
GCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAGA232220.10239466923351492No Hit
CGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATC231880.10224475024488607No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCA231090.10189640906542487No Hit
GGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAA230800.10176853698688848No Hit
TGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCA230200.10150397406577871No Hit
TGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCA229910.10137610198724234No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCTGTAGGCACCATC229820.10133641754907588No Hit
CCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCA228100.10057800384189455No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)