FastQCFastQC Report
Tue 27 Jun 2023
SRR1042902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1042902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3818427
Total Bases160.3 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAA1434003.7554731306896794No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCA1313343.439479136304033No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAAT769552.015358680419974No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCAT481491.2609642661755744No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA418881.0969962238377218No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATC417421.0931726598413432No Hit
CGCTGAGAAGACGGTCGAACTTGCTGTAGGCACCATCAATAG398011.042340209725104No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAAT382761.0024022981190948No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATC379020.9926076889776864No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCA316770.8295824432416804No Hit
GGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCA285500.7476900828534891No Hit
GGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAAT269050.7046095159079904No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATA267490.7005240639666543No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC244790.6410755004613156Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCA244650.6407088573383752No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATC214520.5618020195226988No Hit
GGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAA203480.5328895903993974No Hit
AGAATTAAACTCCGAATGCCATAACTGTAGGCACCATCAATA200350.5246924977222296No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCA193780.5074864597385259No Hit
GCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATA179600.4703507491435609No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCAT178740.468098512816927No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAA172840.4526471240644381No Hit
GGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATA164760.43148657811187696No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATC164590.43104136860544934No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCA157980.4137305754437626No Hit
CGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATC151180.3959221951866567No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAA149740.39215100877926956No Hit
CCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAAT147190.3854728661828549No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCA146920.3847657687314698No Hit
CCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAA146510.3836920281571443No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG143740.37643773208182324No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACC143430.3756258794524552No Hit
GGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAG141480.3705190645257851No Hit
CGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCA135630.3551986197457749No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAA131490.3443564588245369No Hit
CCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATCA129840.34003530773273916No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCA119450.31282515025166124No Hit
GGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAA117080.3066184059561699No Hit
GGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAA116740.3057279869433146No Hit
CGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAG112960.2958286226239234No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAA107770.28223663828063233No Hit
GGGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATC104260.2730443714126262No Hit
TCTTGAGTTGTGATGCATAGTGACTGTAGGCACCATCAATAG102520.2684875211703667No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAA101180.2649782227079371No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA100530.2632759510657137No Hit
GCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAGA97850.2562573541408543No Hit
CTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAAT97730.2559430886069054No Hit
AGCTTCCTCAAAGATGAGTATTCCTCTGTAGGCACCATCAAT94300.24696033209486526No Hit
GCTGAGAAGACGGTCGAACTTGCTGTAGGCACCATCAATAGA94300.24696033209486526No Hit
GCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAAT86230.2258259749367999No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG85700.22443796882852546No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCA84180.22045727206517238No Hit
CTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATCAA83940.2198287409972745No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATC82550.21618849856236613No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGCTGTAGGCACCATCA81270.21283633286691092No Hit
GGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAAT79960.20940560078796844No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAAT79330.2077557067347366No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCAT78890.20660339977692385No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACC76650.20073710980987722No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCA76390.20005620115298786No Hit
TCGCTGAGAAGACGGTCGAACTTGCTGTAGGCACCATCAATA75130.19675641304652414No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCA75020.19646833630707095No Hit
CCCCCCCGAGTGTTACAGCCCCCCTGTAGGCACCATCAATAG72140.1889259634922967No Hit
CCCCCCCGAGTGTTACAGCCCCCCCTGTAGGCACCATCAATA71730.18785222291797118No Hit
CGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGA70180.1837929597711309No Hit
GCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCGGAAGA66750.17481020325909072No Hit
TAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAG66170.17329125317833757No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAA65570.17171992550859294No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAA65100.170489052167293No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTCTGTAGGCACCATC64940.17007003145536106No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCA64600.16917961244250576No Hit
CGCTGAGAAGACGGTCGAACTTGACTGTAGGCACCATCAATA61960.1622657706956294No Hit
TCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATA60690.1589397937946699No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60510.15846839549374653No Hit
CGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAATA60120.1574470325084125No Hit
CTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATA59600.1560852151946338No Hit
GCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAG59500.15582332724967637No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAA58910.15427818837442747No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCA58900.15425199957993174No Hit
GGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCA57670.15103077785695523No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCAT55820.1461858508752426No Hit
TGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCA55140.14440501284953203No Hit
GCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAATAG54520.1427813075907959No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACC54300.14220515411188953No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCAC53040.1389053660054258No Hit
CCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAA51800.13565795548795354No Hit
TAATCAAACTCCGAATACCATTAACTGTAGGCACCATCAATA50990.1335366631337983No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATTAA49040.12842984820712824No Hit
TGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAA48130.12604666790801553No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGCTGTAGGCACCATC47960.12560145840158787No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATC47610.12468485059423684No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATC47200.12361111001991133No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCA47040.12319208930797944No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAG46800.12256355824008158No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATC46660.12219691511714116No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA46430.12159457284373906No Hit
CACAATACCGAAGCTACGGGCACATCTGTAGGCACCATCAAT45610.11944709169508805No Hit
CCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCA45510.11918520375013061No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCA44940.11769244246387321No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAAT44110.11551877252072647No Hit
GCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCA43050.11274276030417761No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCCTGTAGGCACCATCAAT42850.11221898441426273No Hit
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATA42630.11164283093535636No Hit
GGGGCGCGGGACATGTGGCGTACGGCTGTAGGCACCATCAAT42160.1104119575940564No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA41550.10881444112981602No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCA40070.10493849954444592No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC39620.10376000379213744No Hit
AGGCACAGCTGGGTAGCTAAGTACGGAAACTGTAGGCACCAT39530.10352430464167575No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCATCATCAA39270.1028433959847864No Hit
CCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCA39190.10263388562882045No Hit
TGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAA38450.1006959148361354No Hit
CCCCCCCGAGTGTTACAGCCCCCCCCTGTAGGCACCATCAAT38440.10066972604163965No Hit
CTCGCCCGTCACGCACCGCACGTTCGTCTGTAGGCACCATCA38270.10022451653521201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)