FastQCFastQC Report
Fri 30 Jun 2023
SRR10446723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73277086
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length50-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1372959718.73654883055803No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC51016086.962078159057798No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT35233594.808268440150582No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC28968583.953293120853632No Hit
CGATACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC10705481.4609587504612287No Hit
CGCCTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC7640641.0427052189275103No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC6241370.8517492084769855RNA PCR Primer, Index 2 (100% over 50bp)
CCGATACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA4206930.5741126223278038No Hit
CCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTT3098920.4229043714975238No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG2634000.3594575253715738No Hit
AGCCTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2352160.3209952972201979No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTA2328390.31775144552009066No Hit
ATCCTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2164940.2954457004472039No Hit
TAACTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1926660.2629280318270298No Hit
CTCCTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1807520.24666919751694275No Hit
CGCGCCCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1731810.23633718185791394No Hit
CACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1656180.2260160836635889No Hit
CCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCT1572550.21460323899888706No Hit
TTCCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1522700.20780029380535137No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA1403550.19154009481217635No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG1382020.18860193212377468No Hit
CGATCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1358330.18536899788837127No Hit
CTACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1357910.1853116811986765No Hit
CGCTCCCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1334090.18206100608312945No Hit
AGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1305010.17809250766330964No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAG1296500.1769311623554463No Hit
TGGACTTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC1290650.17613282274898323RNA PCR Primer, Index 2 (98% over 50bp)
ACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCT1278510.17447609748018636No Hit
CGAGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1271010.17345258516420808No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTA1253890.17111624771760167No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1163350.1587604070391118No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1122600.15319932345562978No Hit
CGCTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1120560.15292092810568367No Hit
CGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTA1075790.1468112419208373No Hit
GACTATTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT992810.13548710165685354No Hit
ATACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC976770.13329814998374798No Hit
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC947960.1293664980073034RNA PCR Primer, Index 2 (100% over 42bp)
TACCAGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA943560.12876603744859616No Hit
CGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTA928760.126746306478399RNA PCR Primer, Index 2 (100% over 47bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTAT928430.12670127193649594No Hit
TACGTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA927200.1265334159166755No Hit
CGACTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA876320.11958990836507882No Hit
CTCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC848440.11578517191581554No Hit
TGCCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA818780.11173752187689341No Hit
TACCCGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA792970.10821527482684012No Hit
ACCCTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC779080.10631973001764836No Hit
AACCTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC761920.10397793383869003No Hit
TACCTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC758900.10356579954612277No Hit
CGCGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC754870.10301583226167044RNA PCR Primer, Index 2 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)