FastQCFastQC Report
Fri 30 Jun 2023
SRR10446728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38885947
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length18-45
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC19013484.889550458935718No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGAT10575882.719717742761929No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT7789822.003248114286634No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC6992341.7981663144271631No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATC6223891.6005499364590503No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAT4500721.1574155568334237No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT4113561.0578525964662762No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG3537620.9097425350088555No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC3422790.8802125868247468No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTC3357630.8634558906331894No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATC3280270.8435618142461594No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGC3017400.7759615575261675No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC2804270.7211525541605043No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC2644250.6800014411375914No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAT2548390.6553498619951316No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCT2282980.5870964130049346No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2239730.5759741430496729No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACG2134530.5489206679214987No Hit
TGGTGACTCTAGATAACCTCGGGCCGAT1993570.5126710685482342No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCG1915770.49266384074431824No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT1868300.48045634583619634No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT1801560.4632933331930942No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT1600800.41166542761579145No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1576370.4053829523555129No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1528570.39309059388472656No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1516280.3899300690812545No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTC1434410.36887619067114397No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1355550.34859637081745753No Hit
TGGTGACTCTAGATAACCTCGGGCCGATC1353810.348148908396136No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATC1348440.3467679467855058No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGC1326590.3411489502878765No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1239050.3186369615738045No Hit
CGTTGGTGACTCTAGATAACCTCGGGCCGATCGC1227700.3157181693427705No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGT1183520.3043567384381818No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGA1176390.30252317116000804No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCG1172710.3015768138551441No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG972430.25007234618717145No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT967700.248855968455648No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAG937540.2410999531527418No Hit
AGATAACCTCGGGCCGATCGCACGCCCC892460.22950707616815916No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCC864010.22219080841724131No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC860740.22134988765993022No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGAT854810.2198249151550816No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCCCCG851770.2190431417293245No Hit
CTTTGGTGACTCTAGATAACCTCGGGCC803950.20674564001231605No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCT789270.2029704972853046No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC760400.19554622136372302No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC756330.1944995707575284No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG751340.19321633082511785No Hit
CGGGCCGCCCCTCCCACGGCGCGACCGCTCT739940.19028468047852864No Hit
GTGGTGACTCTAGATAACCTCGGGCCGATCGC735580.18916345280211383No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTCCGCCT726640.18686442174084122No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTC725730.18663040403773629No Hit
CGTTGGTGACTCTAGATAACCTCGGGCCGAT695990.1789823969054939No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCG652780.16787041344267634No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG652660.16783955396534384No Hit
GGTGACTCTAGATAACCTCGGGCCGAT650290.1672300792880266No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC629810.16196339515661018No Hit
GGGTGACTCTAGATAACCTCGGGCCGATCGC622640.16011954138599221No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATC620170.15948435047756457No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC612120.1574141938731748No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC604240.15538775486167278No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT602450.15492743432479605No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC602330.15489657484746353No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCAC600970.15454683410436168No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGC598740.15397336215059904No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTC598040.153793348532826No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGC596680.15344360778972413No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC595030.15301928997640202No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT594140.15279041551951916No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCC588450.15132716196933563No Hit
GTGGTGACTCTAGATAACCTCGGGCCGAT558900.14372801567620302No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGA558160.14353771556598582No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC557840.14345542362643246No Hit
GAAGCCGAGCGCACGGGGTCGGCGGCGAT540030.13887536286566457No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC539520.13874421008700136No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCC536820.1380498718470197No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTCCG531810.13676148866838708No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG529960.13628573839284408No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC523820.13470676180266358No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATG523630.13465790096355373No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT516300.1327729012231591No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCC515620.13259803085160815No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG515430.13254917001249833No Hit
CGTCGCGCCGTCGGGTCCCGGGGG511190.13145880181341604No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACG499880.1285502960748262No Hit
TTGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT499740.1285142933512716No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAA477570.12281300491408889No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC474180.12194122467944525No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCT473310.12171749346878451No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCC468190.12040082243593038No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGG459210.11809150488221362No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC456810.11747431533556324No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC434870.11183217422993452No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGC434670.11178074176771367No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC415300.10679950780162303No Hit
GGGTGACTCTAGATAACCTCGGGCCGAT410680.10561141792432109No Hit
CGTTGGTGACTCTAGATAACCTCGGGCCGATC407760.10486050397589648No Hit
CGCCCCTCCCACGGCGCGACCGCTCT398420.10245860799018215No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC391800.10075619349067158No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGT391100.10057617987289855No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCC389450.10015186205957644No Hit
CTGTGGTGACTCTAGATAACCTCGGGCCGATCGC389220.10009271472802245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)