FastQCFastQC Report
Fri 30 Jun 2023
SRR10446731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52196826
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length18-45
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC23587904.5190295670468545No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC22084734.2310484549386205No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC7963191.5256080896566393No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG6569041.2585133050044077No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC6524771.2500319463869318No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT6113801.1712972738993748No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACG5747571.1011340038185464No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC5199500.9961333664234681No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT5038330.9652560100110301No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCC4830190.9253800221492395No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATC4399570.8428807529408013No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCT4182980.8013858926977667No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC3686560.7062804929939609No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT3665040.7021576369413726No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCG3410460.6533845563713012No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGAT3393990.6502291920968527No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC3119530.5976474508239256No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT2974330.5698296674207739No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT2545950.48775954308026315No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCCCCG2405460.4608441133949409No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2313320.4431916990508196No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC2210070.42341080279479065No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG2147620.41144647377601085No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC2093220.4010243841263451No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTCCGCCT2085100.39946873398010835No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAG2072230.39700306681482894No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1712860.3281540528920283No Hit
CGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC1710510.3277038339457652No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC1706340.32690493479431104No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC1631990.3126607736646669No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCC1612240.30887701869075335No Hit
CGTTGGTGACTCTAGATAACCTCGGGCCGATCGC1601660.3068500755199176No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC1579020.3025126470333656No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCC1553500.2976234608594783No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT1455500.27884837288765413No Hit
ACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCG1311780.251314131629383No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAC1308700.2507240574359828No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATG1234250.23646073805330617No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCG1212730.2323378820007178No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCC1194530.2288510799488076No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTCCG1166120.22340822026228183No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGC1120040.21458009726491797No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1044980.20019991253874325No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTC1040930.19942400329092808No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1024560.19628779726951215No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCC1012060.19389301564045291No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG981780.18809189662221992No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA971130.18605154267426147No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAC951850.18235783148960052No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCC936810.1794764302335165No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT932630.1786756152567591No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTC922220.1766812411160786No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGA919810.17621952721799597No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT918800.17602602886236798No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCC906690.17370596442013544No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT899110.17225376884027394No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAA879530.16850258289651557No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC830310.15907289075393205No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCAC829990.15901158434422813No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC788390.15104175108271908No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG786080.15059919543766895No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCT763140.1462042921920195No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC752720.14420800222603572No Hit
ACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGG734990.14081124396337816No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT726050.139098496142275No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC714780.13693936102551524No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC708780.1357898658435668No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC704970.13505993640302955No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCG694110.13297935012370293No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC679500.13018032935565854No Hit
TCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTC673280.12898868601703867No Hit
ACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCTG672010.12874537620352625No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCT668290.12803268919071822No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC646450.12384852672842597No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC643090.12320480942653488No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC582730.11164088789613377No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC579370.11099717059424265No Hit
CGTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC578280.1107883456361887No Hit
AAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCG577110.11056419407570874No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC553420.10602560393231572No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGT541620.10376493007448384No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCC530360.10160771078302731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)