FastQCFastQC Report
Fri 30 Jun 2023
SRR10446732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84021657
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length18-44
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT10848811.2911921030074425No Hit
ATGCGTGCATTTATCAGATCAAAACCAACC6888200.8198124443082574No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTC6248740.7437058757362998No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC5355430.637386858485783No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC5188830.6175586372927637No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT5098810.6068447329002331No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5081190.6047476545243567No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCC4413170.5252419623193102No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCC4063350.4836074584913268No Hit
ATGCGTGCATTTATCAGATCAAAACCAA3939090.46881841428097515No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGT3750840.44641347646833485No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCT3630150.4320493227121193No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCG3388900.4033364874010995No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT3249910.3867943237539341No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC3034600.3611687877091022No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC3016170.3589753056167412No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC2999020.3569341651998127No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC2842490.3383044445314855No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT2614940.31122214121532976No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTC2547360.3031789768202262No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCC2499870.29752686262781036No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT2453460.2920032867240407No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT2404710.28620121119487085No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2301920.2739674605560326No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2178950.25933194819045285No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCC2171620.2584595540647336No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2137780.25443202102048523No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCG2122520.2526158226086877No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCG2004830.23860871965426722No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCT1804060.21471368982880212No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCC1774620.2112098312938532No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCC1729420.2058302658801409No Hit
ATGCGTGCATTTATCAGATCAAAACC1700730.20241567004564073No Hit
ATGCGTGCATTTATCAGATCAAAACCAAC1677670.199671139549176No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCC1631210.19414161279870973No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1581390.18821218915023302No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1534710.1826564786743018No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC1527880.18184359301554837No Hit
CCGGTGGGGGGCGGGGCGGACTGTC1484590.17669134994564556No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT1382280.1645147274350945No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC1362800.1621962775621052No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCC1273880.15161329179689945No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1226920.14602425657946735No Hit
ACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCG1204900.14340350369429156No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1195020.1422276163870465No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGT1160590.13812986335177846No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTCCGCCT1123770.1337476598444137No Hit
CGGTGGGGGGCGGGGCGGACTGTC1115130.1327193535352439No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1109170.13201001260901105No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTC1079270.12845140628445353No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTC1046040.12449647356990352No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1040040.1237823719663134No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGC1036580.12337057337490975No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCT1034160.12308255239479507No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAA1013260.12059509847562279No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTCCG1000850.11911809832553052No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT999560.11896456648075864No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCCCCG997630.11873486379827049No Hit
CCCGGTGGGGGGCGGGGCGGACTGTC991380.11799100796119744No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTCC983550.11705910536851231No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCC978790.11649258476299747No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGT933420.11109278647051675No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCC910100.10831731157122979No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT901450.10728781509272067No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACG892180.10618452811517393No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTC883700.1051752645154332No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAC874500.10408030872326166No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAA848750.10101562267452069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)